Identifying the alien chromosomes in wheat - Leymus multicaulis derivatives using GISH and RFLP techniques

文献类型： 外文期刊

第一作者： Jia, JZ

作者： Jia, JZ;Zhou, RH;Li, P;Zhao, ML;Dong, YS

作者机构：

关键词： in situ hybridization;Leymus multicaulis;RFLP;wheat

期刊名称：EUPHYTICA （ 影响因子：1.895； 五年影响因子：2.181 ）

ISSN： 0014-2336

年卷期： 2002 年 127 卷 2 期

页码：

收录情况： SCI

摘要： Genomic in situ hybridization (GISH) and restriction fragment length polymorphism (RFLP) were used to identify the Leymus multicaulis (XXNN, 2n = 28) chromosomes in wheat- L. muliticaulis derivatives. Fifteen lines containing L. multicaulis alien chromosomes or chromosomal fragments were identified. All alien chromosomes or fragments in these 15 lines were from the X genome and none were from the N genome. Eleven L. multicaulis disomic addition lines and four translocation-addition lines were identified with chromosome rearrangements among homoeologous groups 2, 3, 6 and 7. Only homoeologous group 1 lacked rearrangements in addition or translocation chromosomes. The results revealed that translocation in non-homoeologous chromosomes widely exists in the Triticeae and therefore it is necessary to identify the alien chromosomes (segments) in a wheat background using these combined techniques. During the course of the work, probe PSR112, was found to detect X genome addition lines involving L. multicaulis chromosomes. This may prove to be a valuable probe for the identification of alien chromosomes in a wheat background.

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