Effect of 5 '-deletion of Arabidopsis profilin2 promoter on its vascular specific expression

文献类型： 外文期刊

第一作者： Liu, YH

作者： Liu, YH;Wang, ZX;Jia, SR

作者机构：

关键词： profilin2;promoter;Kalanchoe;constitutive expression;vascular-specific expression

期刊名称：CHINESE SCIENCE BULLETIN （ 影响因子：1.649； 五年影响因子：1.738 ）

ISSN： 1001-6538

年卷期： 2001 年 46 卷 19 期

页码：

收录情况： SCI

摘要： Based on the published sequence of profilin2 promoter of Arabidopsis thaliana, a full-length promoter (1667 bp) was amplified by PCR. The 5'-end deletion fragments with length of 1380, 1153, 969 and 597 bp were then fused with gus (uidA) gene respectively. Constructed plant expression vectors were individually transferred into Kalanchoe laciniata and transgenic plants regenerated. GUS histochemical assay confirmed that the full-length promoter Pfn1.7 was vascular-specific. Deletion assays showed that profilin2 promoter could be divided into three parts. Deletion of fragment 1 (-1667 - -1380 bp) resulted in constitutive expression, suggesting that element(s) responsible for vascular-specific expression might exist in this region. Fragment 2 located at -1153 - -597 lip strongly inhibited gus gene expression. Fragment 3 ( -597 - -1 bp) is considered as a basic domain of profilin2.

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