An improved method of fluorescent labeled amplified fragment length ploymorphism
文献类型: 外文期刊
第一作者: Yang, K
作者: Yang, K;Song, W;Zhang, CQ;Jia, JZ
作者机构:
关键词: auto-sequencer;fluorescent labeled;amplified fragment polymorphism (AFLP)
期刊名称:PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS ( 影响因子:0.351; 五年影响因子:0.272 )
ISSN: 1000-3282
年卷期: 2001 年 28 卷 2 期
页码:
收录情况: SCI
摘要: A new and efficient reaction system has been set up,, in which the Mse I primers were fluorescent labeled for auto-sequencer. PCR reagents and printers and adapters of Mse T and EcoR T, which were synthesized, the AFLP protocol has been modified, and reaction and electrophoresis conditions were optimized, the results obtained can be comparable to that of AFLP fluorescent labeled AFLP kits with less cost.
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