文献类型： 外文期刊

第一作者： Xie, DY

作者： Xie, DY;Zou, ZR;Ye, HC;Li, GF;Guo, ZC

作者机构：

期刊名称：ISRAEL JOURNAL OF PLANT SCIENCES （ 影响因子：0.721； 五年影响因子：1.022 ）

ISSN： 0792-9978

年卷期： 2001 年 49 卷 2 期

页码：

收录情况： SCI

摘要： Hairy roots were induced from two kinds of explants and selection of hairy root clones was studied for artemisinin production. Leaf blade pieces and petiole segments of Artemisia annua plantlets were infected with Agrobacterium rhizogenes strain 1601. The efficiency of leaf blade pieces forming hairy roots was higher than that of petiole segments. Light promoted hairy root induction and branching. Two hundred and twenty hairy root clones showed considerable variations in capacity of growth and branching. Six hairy root clones were established in suspension culture and showed obvious differences in their biomass and artemisinin content. Among six clones, clone 1601-L-3 produced the highest biomass, more than 70 times the inoculum, while clone 1601-L-1 gave the lowest biomass. The artemisinin content of clone 1601-L-1 was the highest, 1.195 mg/g DW, and this line yielded the highest artemisinin level of 9.08 mg/L.

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