Identification of molecular markers for the Thinopyrum intermedium chromosome 2Ai-2 with resistance to barley yellow dwarf virus

文献类型： 外文期刊

第一作者： Zhang, ZY

作者： Zhang, ZY;Xin, ZY;Lin, ZS;Chen, X;Wang, XP

作者机构：

关键词： barley yellow dwarf virus (BYDV);Thinopyrum intermedium;addition lines;genomic in situ hybridization (GISH);RFLP;RAPD

期刊名称：ACTA BOTANICA SINICA （ 影响因子：0.599； ）

ISSN： 0577-7496

年卷期： 2000 年 42 卷 10 期

页码：

收录情况： SCI

摘要： The wheat-Thinopyrum intermedium addition lines Z1, Z2 contain a pair of Th. intermedium chromosomes 2Ai-2 carrying the gene with resistance to barley yellow dwarf virus (BYDV). Genomic in situ hybridization (GISH) was used to analyze the chromosome constitution of Z1, Z2 by using genomic DNA probes from th. intermedium and Pseudoroegneria strigosa. The results showed that the chromosome constitution of either Z1 or Z2 composes of 42 wheat chromosomes and two Th. intermedium chromosomes (2Ai-2). The 2Ai-2 chromosome is St-E intercalary translocation, in which the E genomic chromosome segment translocated into the middle region of the long arm of chromosome belonging to St genome. With the genomic DNA probe of Ps. strigosa, the GISH pattern specific to the 2Ai-2 chromosome may be used as a molecular cytogenetic marker. A detailed RFLP analysis on Z1, Z2 and their parents was carried out by using 12 probes on the wheat group 2 chromosomes. Twenty RFLP markers specific to the 2Ai-2 chromosome were identified. Two RAPD markers of OPR16(-350) and OPH09(-1580), specific to the 2Ai-2 chromosome, were identified from 280 RAPD primers. These molecular markers could be used to assisted-select translocation lines with small segment of the 2Ai-2 chromosome and provide tools to localize the BYDV resistance.

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