Integrin-like proteins in the pollen tube: Detection, localization and function

文献类型： 外文期刊

第一作者： Sun, Y

作者： Sun, Y;Qian, H;Xu, XD;Han, Y;Yen, LF;Sun, DY

作者机构：

关键词： immunochemical detection/localization;integrin-like protein (subunits);pollen tube;tip growth

期刊名称：PLANT AND CELL PHYSIOLOGY （ 影响因子：4.927； 五年影响因子：5.516 ）

ISSN： 0032-0781

年卷期： 2000 年 41 卷 10 期

页码：

收录情况： SCI

摘要： The distribution of integrin-like proteins in the pollen tube was examined by immunofluorescent labeling and western blotting techniques using antibodies against human placenta integrin vitronectin receptor (VnR), and alpha (v) beta (3) and beta (1) integrin subunits, Pseudocolor-coded confocal images showed intense immunostaining within 10 and 5 mum of the tip of the pollen tube in Lilium davidii and Nicotiana tabacum respectively, In both segments the site near the plasma membrane was labeled. Western blotting analyses revealed cross-reaction of anti-beta (3) anti-alpha (v) and anti-VnR with the proteins in the plasma membrane preparation of L, davidii and Hemerocallis citrina pollen tube. These studies provide evidence for the first time that the integrin-like protein is present in pollen tubes, and it may be mainly composed of alpha (v) and beta (3) subunits in Lily pollen tubes. In a functional assay, neither anti-VnR antibody nor the Arg-Gly-Asp-Ser tetrapeptide inhibited pollen tube growth of N, tabacum in vitro, but both of them depressed tube growth on the stigma and in style under quasi in vivo culture conditions. The integrin-like proteins localized in the tip and periphery of the pollen tube appeared to play roles in growth of the pollen tube tip and interaction with the extracellular matrix of the style.

分类号：

  • 相关文献

[1]The ARID-HMG DNA-binding protein AtHMGB15 is required for pollen tube growth in Arabidopsis thaliana. Xia, Chuan,Wang, Yu-Jiao,Liang, Yan,Niu, Qian-Kun,Tan, Xiao-Yun,Chu, Liang-Cui,Chen, Li-Qun,Zhang, Xue-Qin,Ye, De,Xia, Chuan,Ye, De.

[2]PwHAP5, a CCAAT-binding transcription factor, interacts with PwFKBP12 and plays a role in pollen tube growth orientation in Picea wilsonii. Huang, Guixue,Zhang, Dun,Wei, Jing,Zhang, Lingyun,Yu, Yanli,Li, Yanze,Yan, Kang,Yu, Yanli,Meng, Zhaodong,Zheng, Chengchao. 2011

[3]Pollen Tube Behavior in Inter-specific Cross between Prunus Armeniaca L. and Prunus Amygdalus Batsch.. Sun Haoyuan,Wang Yuzhu,Yang Li,Cai Shengwen. 2011

[4]Malus domestica ADF1 severs actin filaments in growing pollen tubes. Yang, Qing,Wang, ShengNan,Wu, ChuanBao,Zhang, QiuLei,Zhang, Yi,Li, Yang,Hao, Li,Gu, Zhaoyu,Li, Wei,Li, Tianzhong,Chen, QiuJu.

作者其他论文 更多>>

微信小程序