Molecular characterisation of the Theileria buffeli/orientalis group

文献类型: 外文期刊

第一作者: Gubbels, MJ

作者: Gubbels, MJ;Hong, Y;van der Weide, M;Qi, B;Nijman, IJ;Guangyuan, L;Jongejan, F

作者机构:

关键词: Theileria buffeli;Theileria orientalis;Theileria sergenti;phylogeny;18S rRNA;major piroplasm surface protein

期刊名称:INTERNATIONAL JOURNAL FOR PARASITOLOGY ( 影响因子:3.981; 五年影响因子:4.214 )

ISSN: 0020-7519

年卷期: 2000 年 30 卷 8 期

页码:

收录情况: SCI

摘要: Benign bovine Theileria parasites known as either Theileria buffeli, Theileria orientalis or Theileria sergenti are classified on basis of their morphology, vector specificity, pathogenicity anti 18S small subunit ribosomal RNA or major piroplasm protein (MPSP) sequences. Since most isolates have been characterized on only some of these criteria and the existing confusion in nomenclature, an analysis was performed on eight different isolates to combine 18S rRNA data with MPSP data and the results were compared with available biological parameters. A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) approach for both genes was used in combination with reverse line blot hybridisation For the 18S rRNA gene. Both MFSP and 18S rRNA genes were cloned and sequenced from parasites displaying aberrant MFSP RFLP profiles. Phylogeny based on published and determined 18S rRNA and MPSP sequences did correlate within the same isolate but there was no obvious correlation between molecular and biological data. Based on these findings, we suggest that the appropriate name for all these parasites is Theileria buffeli. A more specific nomenclature should be assigned when new molecular markers may become available. (C) 2000 Australian Society for Parasitology Inc. Published by Elsevier Science Ltd. All rights reserved.

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