ORF1a of highly pathogenic PRRS attenuated vaccine virus plays a key role in neutralizing antibody induction in piglets and virus neutralization in vitro

文献类型: 外文期刊

第一作者: Leng, Chaoliang

作者: Leng, Chaoliang;Zhang, Wuchao;Zhang, Hongliang;Li, Zhen;Liu, Chunxiao;An, Tongqing;Peng, Jinmei;Wang, Qian;Cai, Xuehui;Tian, Zhijun;Tong, Guangzhi;Leng, Chaoliang;Kan, Yunchao;Yao, Lunguang;Zhai, Hongyue;Li, Mingliang;Leng, Yumin;Tong, Guangzhi

作者机构:

关键词: PRRSV;Chimeric virus;Neutralizing antibody;Neutralization region

期刊名称:VIROLOGY JOURNAL ( 影响因子:4.099; 五年影响因子:3.719 )

ISSN: 1743-422X

年卷期: 2017 年 14 卷

页码:

收录情况: SCI

摘要: Background: Currently, porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most economically important viral pathogens in swine in most countries, especially China. Two PRRSV attenuated live vaccine strains (HuN4-F112 and CH-1R) are currently widely used in China. Our previous study showed that HuN4-F112, but not CH-1R, induced high anti-nucleocapsid (N) antibody and neutralizing antibody (NA) titers. Additionally, sera from HuN4-F112 inoculated pigs induced low cross neutralization of CH-1R. Methods: In the present study, 6 chimeric viruses through exchanging 5' untranslated region (UTR) + open reading frame (ORF) 1a, ORF1b, and ORF2-7 + 3'UTR between HuN4-F112 and CH-1R were constructed and rescued based on the infectious clones of rHuN4-F112 and rCH-1R. The characteristics of these viruses were investigated in vitro and vivo. Results: All the three fragments, 5' UTR + ORF1a, ORF1b, and ORF2-7 + 3'UTR, could affect the replication efficiencies of rHuN4-F112 and rCH-1R in vitro. Additionally, both 5' UTR + ORF1a and ORF2-7 + 3'UTR affected the anti-N antibody and NA responses targeting rHuN4-F112 and rCH-1R in piglets. Conclusions: The 5' UTR + ORF1a region of HuN4-F112 played a key role in inducing NAs in piglets. Furthermore, we confirmed for the first time that ORF1a contains a neutralization region. This study provides important information that can be used for further study of the generation of anti-PRRSV NAs.

分类号:

  • 相关文献

[1]Identification of a peptide derived from the heptad repeat 2 region of the porcine epidemic diarrhea virus (PEDV) spike glycoprotein that is capable of suppressing PEDV entry and inducing neutralizing antibodies. Zhao, Pengwei,Wang, Ming,Zhao, Pengwei,Wang, Bin,Ji, Chun-Miao,Huang, Yao-Wei,Zhao, Pengwei,Wang, Bin,Ji, Chun-Miao,Huang, Yao-Wei,Cong, Xiaoyan. 2018

[2]Porcine epidemic diarrhea virus virus-like particles produced in insect cells induce specific immune responses in mice. Wang, Cuiling,Wang, Cuiling,Yan, Feihu,Zheng, Xuexing,Wang, Hualei,Jin, Hongli,Wang, Chong,Zhao, Yongkun,Feng, Na,Wang, Tiecheng,Gao, Yuwei,Yang, Songtao,Xia, Xianzhu,Zheng, Xuexing,Jin, Hongli,Wang, Chong,Zheng, Xuexing,Wang, Hualei,Zhao, Yongkun,Feng, Na,Wang, Tiecheng,Gao, Yuwei,Yang, Songtao,Xia, Xianzhu.

[3]Evidence of VP1 of duck hepatitis A type 1 virus as a target of neutralizing antibodies and involving receptor-binding activity. Li, Xiaojun,Zhao, Ran,Li, Chenxi,Zhang, Tingting,Meng, Fanyi,Liu, Ming,Zhang, Yun,Lin, Wei.

[4]A spike-specific whole-porcine antibody isolated from a porcine B cell that neutralizes both genogroup 1 and 2 PEDV strains. Fu, Fang,Li, Lin,Shan, Lingling,Yang, Beibei,Shi, Hongyan,Zhang, Jiaoer,Feng, Li,Liu, Pinghuang,Wang, Hongfeng.

[5]Newcastle disease virus-vectored West Nile fever vaccine is immunogenic in mammals and poultry. Wang, Jinliang,Yang, Jie,Ge, Jinying,Hua, Ronghong,Liu, Renqiang,Wang, Xijun,Shao, Yu,Sun, Encheng,Wu, Donglai,Wen, Zhiyuan,Bu, Zhigao,Li, Xiaofeng,Qin, Chengfeng. 2016

[6]Nanoparticle orientationally displayed antigen epitopes improve neutralizing antibody level in a model of porcine circovirus type 2. Ding, Peiyang,Li, Yafei,Zhou, Enmin,Zhang, Gaiping,Ding, Peiyang,Zhang, Teng,Li, Yafei,Teng, Man,Sun, Yaning,Liu, Xiao,Chai, Shujun,Jin, Qianyue,Zhang, Gaiping,Zhang, Teng,Liu, Xiao,Zhang, Gaiping,Jin, Qianyue,Zhang, Gaiping. 2017

[7]Human papillomavirus L1 protein expressed in Escherichia coli self-assembles into virus-like particles that are highly immunogenic. Chen, Yumei,Zhang, Gaiping,Dong, Ziming,Chen, Yumei,Liu, Yunchao,Zhang, Gaiping,Zhang, Gaiping,Wang, Jucai,Zhao, Baolei,Wang, Aiping,Qi, Yanhua,Li, Ning,Jiang, Min.

[8]Localization, Expression Change in PRRSV Infection and Association Analysis of the Porcine TAP1 Gene. Sun, Nunu,Liu, Dewu,Meng, Fanming,Zhang, Xianwei,Wu, Zhenfang,Sun, Nunu,Liu, Xiangdong,Xie, Shengsong,Li, Xinyun,Sun, Nunu,Liu, Xiangdong,Xie, Shengsong,Li, Xinyun,Chen, Hongbo,Chen, Huiyong. 2012

[9]Porcine reproductive and respiratory syndrome virus as a vector: Immunogenicity of green fluorescent protein and porcine circovirus type 2 capsid expressed from dedicated subgenomic RNAs. Du, Yijun,Song, Cheng,Yoo, Dongwan,Pei, Yanlong,Hodgins, Douglas C.,Wu, Jiaqiang,Song, Cheng,Welch, Siao-Kun W.,Calvert, Jay G.,Li, Gang. 2009

[10]Identification of immunodominant T-cell epitopes in membrane protein of highly pathogenic porcine reproductive and respiratory syndrome virus. Tong, Guang-zhi. 2011

[11]Antigenic structure analysis of glycosylated protein 3 of porcine reproductive and respiratory syndrome virus. Zhou, YJ,An, TQ,He, YX,Liu, JX,Qiu, HJ,Wang, YF,Tong, G. 2006

[12]Generation of an infectious clone of HuN4-F112, an attenuated live vaccine strain of porcine reproductive and respiratory syndrome virus. Zhang, Shanrui,Zhou, Yanjun,Jiang, Yifeng,Li, Guoxin,Yan, Liping,Yu, Hai,Tong, Guangzhi. 2011

[13]Interference of porcine reproductive and respiratory syndrome virus replication on MARC-145 cells using DNA-based short interfering RNAs. He, Yun-xia,Hua, Rong-hong,Zhou, Yan-jun,Qiu, Hua-ji,Tong, Guang-zhi. 2007

[14]Mapping codon usage of the translation initiation region in porcine reproductive and respiratory syndrome virus genome. Su, Jun-hong,Li, Ji-dong,Ma, Xu-sheng,Dou, Yong-xi,Luo, Xue-nong,Cai, Xue-peng,Ma, Xiao-xia,He, Ya-li,Li, Ji-dong. 2011

[15]Enhanced immunogenicity induced by an alphavirus replicon-based pseudotyped baculovirus vaccine against porcine reproductive and respiratory syndrome virus. Wu, Qunfeng,Xu, Fengqin,Fang, Liurong,Xu, Jinfang,Li, Bin,Jiang, Yunbo,Chen, Huanchun,Xiao, Shaobo,Li, Bin. 2013

[16]The integrity of PRRSV nucleocapsid protein is necessary for up-regulation of optimal interleukin-10 through NF-kappa B and p38 MAPK pathways in porcine alveolar macrophages. Liu, Yanyan,Zhang, Yuyu,Zhu, Xiwang,Ren, Sufang,Guo, Lihui,Sun, Wenbo,Chen, Zhi,Cong, Xiaoyan,Chen, Lei,Du, Yijun,Li, Jun,Wu, Jiaqiang,Wang, Jinbao,Yu, Jiang,Liu, Yanyan,Zhang, Yuyu,Zhu, Xiwang,Ren, Sufang,Guo, Lihui,Sun, Wenbo,Chen, Zhi,Cong, Xiaoyan,Chen, Lei,Shi, Jianli,Du, Yijun,Li, Jun,Wu, Jiaqiang,Wang, Jinbao,Liu, Yanyan,Zhu, Xiwang,Wu, Jiaqiang,Wang, Jinbao,Liu, Xing. 2017

[17]Stable expression of foreign gene in nonessential region of nonstructural protein 2 (nsp2) of porcine reproductive and respiratory syndrome virus: Applications for marker vaccine design. Xu, Yan-Zhao,Zhou, Yan-Jun,Zhang, Shan-Rui,Jiang, Yi-Feng,Tong, Wu,Yu, Hai,Tong, Guang-Zhi.

[18]Influence of N-linked glycosylation of minor proteins of porcine reproductive and respiratory syndrome virus on infectious virus recovery and receptor interaction. Wei, Zuzhang,Tian, Debin,Sun, Lichang,Lin, Tao,Gao, Fei,Liu, Runxia,Tong, Guangzhi,Yuan, Shishan.

[19]Hemagglutinating virus of Japan envelope (HVJ-E) can enhance the immune responses of swine immunized with killed PRRSV vaccine. Dai, Zhihong,Zhang, Zhongqiu,Zhao, Deming,Dai, Zhihong,Wang, Zaishi,Zhang, Quan,Zhang, Zhongqiu,Guo, Pengju. 2011

[20]Production of porcine TNF alpha by ADAM17-mediated cleavage negatively regulates porcine reproductive and respiratory syndrome virus infection. Li, Ren,Guo, Longjun,Gu, Weihong,Luo, Xiaolei,Zhang, Jian,Xu, Yunfei,Tian, Zhijun,Feng, Li,Wang, Yue.

作者其他论文 更多>>