Chemosensory genes in the antennal transcriptome of two syrphid species, Episyrphus balteatus and Eupeodes corollae (Diptera: Syrphidae)

文献类型： 外文期刊

第一作者： Liu, Yang

作者： Liu, Yang;Wang, Gui-Rong

作者机构：

关键词： Episyrphus balteatus;Eupeodes corollae;Transcriptome;Chemosensory genes;Odorant receptors;Syrphid olfaction

期刊名称：BMC GENOMICS （ 影响因子：3.969； 五年影响因子：4.478 ）

ISSN： 1471-2164

年卷期： 2017 年 18 卷

页码：

收录情况： SCI

摘要： Background: Predatory syrphid larvae are an important natural enemy of aphids in cotton agro-ecosystems in China. Their behaviors in prey foraging, localization and oviposition greatly rely on the perception of chemical cues. As a first step to better understand syrphid olfaction at the molecular level, we have performed a systematic identification of their major chemosensory genes. Results: Male and female antennal transcriptomes of Episyrphus balteatus and Eupeodes corollae were sequenced and assembled using Illumina HiSeq2000 technology. A total of 154 chemosensory genes in E. balteatus transcriptome, including candidate 51 odorant receptors (ORs), 32 ionotropic receptors (IRs), 14 gustatory receptors (GRs), 49 odorant-binding proteins (OBPs), 6 chemosensory proteins (CSPs) and 2 sensory neuron membrane proteins (SNMPs) were identified. In E. corollae transcriptome, we identified 134 genes including 42 ORs, 23 IRs, 16 GRs, 44 OBPs, 7 CSPs and 2 SNMPs. We have provided full-length sequences of the highly conserved co-receptor Orco, IR8a/25a family and carbon dioxide gustatory receptor in both syrphid species. The expression of candidate OR genes in the two syrphid species was evaluated by semi-quantitative reverse transcription PCR. There were no significant differences of transcript abundances in the respective male and female antenna, which is consistent with differentially expressed genes (DEGs) analysis using the FPKM value. The sequences of candidate chemosensory genes were confirmed and phylogenetic analysis was performed. Conclusions: This research comprehensively analyzed and identified many novel candidate chemosensory genes regarding syrphid olfaction. It provides an opportunity for understanding how syrphid insects use chemical cues to conduct their behaviors among tritrophic interactions of plants, herbivorous insects, and natural enemies in agricultural ecosystems.

分类号：

  • 相关文献

[1]Candidate chemosensory genes identified in the endoparasitoid Meteorus pulchricornis (Hymenoptera: Braconidae) by antennal transcriptome analysis. Sheng, Sheng,Liao, Cheng-Wu,Zheng, Yu,Zhou, Yu,Xu, Yan,Song, Wen-Miao,Zhang, Jian,Wu, Fu-An,Sheng, Sheng,Zhang, Jian,Wu, Fu-An,He, Peng.

[2]Identification and comparison of candidate odorant receptor genes in the olfactory and non-olfactory organs of Holotrichia oblita Faldermann by transcriptome analysis. Wei, Hongshuang,Shu, Changlong,Zhang, Shuai,Cao, Yazhong,Yin, Jiao,Luo, Chen. 2017

[3]Molecular identification and expression patterns of odorant binding protein and chemosensory protein genes in Athetis lepigone (Lepidoptera: Noctuidae). Zhang, Ya-Nan,Zhu, Xiu-Yun,Xu, Ji-Wei,Zhang, Ya-Nan,Ma, Ji-Fang,Dong, Zhi-Ping,Kang, Ke,Zhang, Long-Wa. 2017

[4]Identification and expression pattern of candidate olfactory genes in Chrysoperla sinica by antennal transcriptome analysis. Zhao-Qun Li,Shuai Zhang,Jun-Yu Luo,Si-BaoWang,Chun-Yi Wang,Li-Min Lv,Shuang-Lin Dong,Jin-Jie Cui.

[5]Functional characterization of pheromone receptors in the tobacco budworm Heliothis virescens. Schal, C.,Gould, F.,Schal, C.,Gould, F.,Wang, G.,Wang, G.,Zwiebel, L. J.,Wang, G.,Zwiebel, L. J.,Wang, G.,Zwiebel, L. J.,Wang, G.,Zwiebel, L. J.,Wang, G.,Zwiebel, L. J..

[6]A collection of 10,096 indica rice full-length cDNAs reveals highly expressed sequence divergence between Oryza sativa indica and japonica subspecies. Liu, Xiaohui,Lu, Tingting,Yu, Shuliang,Li, Ying,Huang, Yuchen,Huang, Tao,Zhang, Lei,Zhu, Jingjie,Zhao, Qiang,Fan, Danlin,Mu, Jie,Shangguan, Yingying,Feng, Qi,Guan, Jianping,Ying, Kai,Zhang, Yu,Lin, Zhixin,Sun, Zongxiu,Qian, Qian,Lu, Yuping,Han, Bin.

[7]Transcriptome analysis of the roots at early and late seedling stages using Illumina paired-end sequencing and development of EST-SSR markers in radish. Wang, Shufen,He, Qiwei,Liu, Xianxian,Xu, Wenling,Li, Libin,Gao, Jianwei,Wang, Fengde,Wang, Xiufeng. 2012

[8]Transcriptome Analysis of Calcium and Hormone-Related Gene Expressions during Different Stages of Peanut Pod Development. Li, Yan,Meng, Jingjing,Yang, Sha,Guo, Feng,Zhang, Jialei,Geng, Yun,Cui, Li,Li, Xinguo,Wan, Shubo. 2017

[9]Transcriptome profiling of peanut gynophores revealed global reprogramming of gene expression during early pod development in darkness. Xia, Han,Zhao, Chuanzhi,Hou, Lei,Li, Aiqin,Zhao, Shuzhen,Bi, Yuping,An, Jing,Wan, Shubo,Wang, Xingjun,Bi, Yuping,Wan, Shubo,Wang, Xingjun,Bi, Yuping,An, Jing,Zhao, Yanxiu,Wang, Xingjun. 2013

[10]Switch on a more efficient pyruvate synthesis pathway based on transcriptome analysis and metabolic evolution. Yang, Maohua,Mu, Tingzhen,Xing, Jianmin,Chen, Ruonan,Zhang, Xiang. 2017

[11]Transcriptome analysis of rosette and folding leaves in Chinese high-throughput RNA sequencing. Wang, Fengde,Li, Libin,Li, Huayin,Liu, Lifeng,Zhang, Yihui,Gao, Jianwei,Wang, Xiaowu. 2012

[12]Transcriptome analysis reveals long noncoding RNAs involved in fiber development in cotton (Gossypium arboreum). Changsong Zou,Qiaolian Wang,Cairui Lu,Wencui Yang,Youping Zhang,Hailiang Cheng,Xiaoxu Feng,Mtawa Andrew Prosper,Guoli Song. 2016

[13]De novo assembly and transcriptome analysis of two contrary tillering mutants to learn the mechanisms of tillers outgrowth in switchgrass (Panicum virgatum L.). Kaijie Xu,Fengli Sun,Guaiqiang Chai,Yongfeng Wang,Lili Shi,Shudong Liu,Yajun Xi. 2015

[14]Global analysis of the Gossypium hirsutum L. Transcriptome during leaf senescence by RNA-Seq. Min Lin,Chaoyou Pang,Shuli Fan,Meizhen Song,Hengling Wei,Shuxun Yu. 2015

[15]PAG1, a cotton brassinosteroid catabolism gene, modulates fiber elongation. Yang, Zuoren,Zhang, Chaojun,Yang, Xiaojie,Liu, Kun,Wu, Zhixia,Zhang, Xueyan,Zheng, Wu,Liu, Chuanliang,Lu, Lili,Yang, Zhaoen,Qian, Yuyuan,Xu, Zhenzhen,Li, Changfeng,Li, Fuguang,Xun, Qingqing,Li, Jia.

[16]Identification of candidate thermotolerance genes during early seedling stage in upland cotton (Gossypium hirsutum L.) revealed by comparative transcriptome analysis. Peng, Zhen,Cao, Moju,Xu, Jie,Lu, Yanli,Peng, Zhen,He, Shoupu,Gong, Wenfang,Sun, Junling,Pan, Zhaoe,Du, Xiongming,Sun, Gaofei.

[17]Characterization of the global transcriptome for cotton (Gossypium hirsutum L.) anther and development of SSR marker. Xianwen Zhang ,Zhenwei Ye,TiankangWang,Hairong Xiong,Xiaoling Yuan,Zhigang Zhang,Youlu Yuan,Zhi Liu.

[18]Transcriptomic Analysis of the Porcine Endometrium during Embryo Implantation. Lin, Haichao,Wang, Huaizhong,Wang, Yanping,Liu, Chang,Wang, Cheng,Guo, Jianfeng,Lin, Haichao,Wang, Yanping,Liu, Chang,Guo, Jianfeng. 2015

[19]De novo assembly of pen shell (Atrina pectinata) transcriptome and screening of its genic microsatellites. Sun, Xiujun,Li, Dongming,Liu, Zhihong,Zhou, Liqing,Wu, Biao,Yang, Aiguo,Sun, Xiujun,Li, Dongming,Liu, Zhihong,Zhou, Liqing,Wu, Biao,Yang, Aiguo. 2017

[20]Identification of Genes Putatively Involved in Chitin Metabolism and Insecticide Detoxification in the Rice Leaf Folder (Cnaphalocrocis medinalis) Larvae through Transcriptomic Analysis. Yu, Hai-Zhong,Wen, De-Fu,Geng, Lei,Xu, Jia-Ping,Wang, Wan-Lin,Zhang, Yan. 2015

作者其他论文 更多>>

微信小程序