Characterization of Monoclonal Antibodies against HA Protein of H1N1 Swine Influenza Virus and Protective Efficacy against H1 Viruses in Mice

文献类型: 外文期刊

第一作者: Liu, Yun

作者: Liu, Yun;Li, Hongtao;Xue, Yujia;Zhao, Shuang;Li, Chenxi;Qu, Liandong;Zhang, Yun;Liu, Ming

作者机构:

关键词: swine influenza virus;HA protein;monoclonal antibodies;protection efficacy

期刊名称:VIRUSES-BASEL ( 影响因子:5.048; 五年影响因子:5.127 )

ISSN: 1999-4915

年卷期: 2017 年 9 卷 8 期

页码:

收录情况: SCI

摘要: H1N1 swine influenza viruses (SIV) are prevalent in pigs globally, and occasionally emerge in humans, which raises concern about their pandemic threats. To stimulate hemagglutination (HA) of A/Swine/Guangdong/LM/2004 (H1N1) (SW/GD/04) antibody response, eukaryotic expression plasmid pCI-neo-HA was constructed and used as an immunogen to prepare monoclonal antibodies (mAbs). Five mAbs (designed 8C4, 8C6, 9D6, 8A4, and 8B1) against HA protein were obtained and characterized. Western blot showed that the 70 kDa HA protein could be detected by all mAbs in MDCK cells infected with SW/GD/04. Three mAbs-8C4, 8C6, and 9D6-have hemagglutination inhibition (HI) and neutralization test (NT) activities, and 8C6 induces the highest HI and NT titers. The protection efficacy of 8C6 was investigated in BALB/c mice challenged with homologous or heterologous strains of the H1 subtype SIV. The results indicate that mAb 8C6 protected the mice from viral infections, especially the homologous strain, which was clearly demonstrated by the body weight changes and reduction of viral load. Thus, our findings document for the first time that mAb 8C6 might be of potential therapeutic value for H1 subtype SIV infection.

分类号:

  • 相关文献

[1]Structure modeling and spatial epitope analysis for HA protein of the novel H1N1 influenza virus. Jin Li,Wang HongYan,Wang XiaoNing,Yang Zhong,Zhong Yang,Zhao GuoPing,Wu Di,Xu TianLei,Sun Jing,Cao ZhiWei,Wu Di,Yang Zhong,Zhong Yang,Li YiXue,Cao ZhiWei,Dai JianXin,Wang Hao,Guo YaJun,Wang XiaoNing,Dai JianXin,Wang Hao,Guo YaJun,Che XiaoYan,Huang Zhong,Lan Ke,Sun Bing,Wu Fan,Yuan ZhenAn,Zhang Xi,Ding GuoHui,He YunGang,Bian Chao,Jin Li,Zhao GuoPing,Li YiXue,Ding GuoHui,He YunGang,Bian Chao,Jin Li,Zhao GuoPing,Li YiXue,Ding GuoHui,He YunGang,Bian Chao,Jin Li,Zhao GuoPing,Li YiXue,Ding GuoHui,He YunGang,Bian Chao,Jin Li,Zhao GuoPing,Li YiXue,Ding GuoHui,He YunGang,Bian Chao,Jin Li,Zhao GuoPing,Li YiXue,Zhou ZhengFeng,Xiong Hui,Dong Hui,Jin WeiRong,Jin Li,Zhao GuoPing,Zhou XiaoNong,Zhou JiaHai,Ma ZhiYong,Tong GuangZhi,Zhao GuoPing. 2009

[2]Genomic Analysis of One Chinese H1N1 Swine Influenza Virus Strain from Healthy Pig Remaining Different Virulence Determinants. Xu Cong,Xue Chun-Yi,Lv Li-Shan,Li Xiao-Ming,Cao Yong-Chang,Shu Ding-Ming,Lin Zhi-Xiong. 2012

[3]The effects of H3N2 swine influenza virus infection on TLRs and RLRs signaling pathways in porcine alveolar macrophages. Zhang, Jinqiu,Hou, Jibo,Zhang, Jinqiu,Miao, Jinfeng,Lu, Chengping. 2015

[4]A recombinant pseudorabies virus encoding the HA gene from H3N2 subtype swine influenza virus protects mice from virulent challenge. Tian, ZJ,Zhou, GH,Zheng, BL,Qiu, HJ,Ni, JQ,Yang, HL,Yin, XN,Hu, SP,Tong, GZ. 2006

[5]Immune efficacy of an adenoviral vector-based swine influenza vaccine against antigenically distinct H1N1 strains in mice. Wu, Yunpu,Wei, Ping,Wu, Yunpu,Yang, Dawei,Xu, Bangfeng,Liang, Wenhua,Sui, Jinyu,Chen, Yan,Yang, Huanliang,Chen, Hualan,Qiao, Chuanling. 2017

[6]Novel triple reassortant H1N2 influenza viruses bearing six internal genes of the pandemic 2009/H1N1 influenza virus were detected in pigs in China. Qiao, Chuanling,Liu, Liping,Yang, Huanliang,Chen, Yan,Xu, Huiyang,Chen, Hualan.

[7]Protective efficacy of a high-growth reassortant swine H3N2 inactivated vaccine constructed by reverse genetic manipulation. Wen, Feng,Ma, Ji-Hong,Yu, Hai,Yang, Fu-Ru,Huang, Meng,Zhou, Yan-Jun,Li, Ze-Jun,Tong, Guang-Zhi.

[8]PA-X protein contributes to virulence of triple-reassortant H1N2 influenza virus by suppressing early immune responses in swine. Xu, Guanlong,Zhang, Xuxiao,Hu, Zhe,Sun, Honglei,Xiong, Xin,Jiang, Ming,He, Qiming,Wang, Yu,Pu, Juan,Guo, Xin,Yang, Hanchun,Liu, Jinhua,Sun, Yipeng,Xu, Guanlong,Zhang, Xuxiao,Hu, Zhe,Sun, Honglei,Xiong, Xin,Jiang, Ming,He, Qiming,Wang, Yu,Pu, Juan,Guo, Xin,Yang, Hanchun,Liu, Jinhua,Sun, Yipeng,Xu, Guanlong,Liu, Qinfang,Bing, Guoxia.

[9]Establishment of a multiplex RT-PCR assay to detect different lineages of swine H1 and H3 influenza A viruses. Fu, Guanghua,Liu, Mengda,Zeng, Wenshu,Pu, Juan,Bi, Yuhai,Liu, Jinhua,Fu, Guanghua,Ma, Guangpeng,Liu, Jinhua. 2010

[10]Pathogenic characteristics of a novel triple-reasserted H1N2 swine influenza virus. Liu, Huili,Tao, Jie,Zhang, Pengchao,Yin, Xiuchen,Ha, Zhuo,Zhang, Chunling,Liu, Huili,Tao, Jie,Yin, Xiuchen.

[11]Isolation and molecular characterization of an H5N1 swine influenza virus in China in 2015. Wu, Haibo,Yang, Fan,Liu, Fumin,Peng, Xiuming,Wu, Nanping,Lu, Rufeng,Xu, Lihua. 2018

[12]Protective efficacy of an inactivated Eurasian avian-like H1N1 swine influenza vaccine against homologous H1N1 and heterologous H1N1 and H1N2 viruses in mice. Sui, Jinyu,Yang, Dawei,Qiao, Chuanling,Xu, Huiyang,Xu, Bangfeng,Wu, Yunpu,Yang, Huanliang,Chen, Yan,Chen, Hualan.

[13]PA-X protein decreases replication and pathogenicity of swine influenza virus in cultured cells and mouse models. Gong, Xiao-Qian,Ruan, Bao-Yang,Liu, Xiao-Min,Wang, Qi,Yang, Hai-Ming,Wang, Shuai-Yong,Zhang, Peng,Wang, Xiu-Hui,Shan, Tong-Ling,Tong, Wu,Zhou, Yan-Jun,Li, Guo-Xin,Zheng, Hao,Tong, Guang-Zhi,Yu, Hai,Sun, Ying-Feng,Shan, Tong-Ling,Tong, Wu,Zhou, Yan-Jun,Li, Guo-Xin,Zheng, Hao,Tong, Guang-Zhi,Yu, Hai.

[14]A novel M2e-multiple antigenic peptide providing heterologous protection in mice. Wen, Feng,Ma, Ji-Hong,Yu, Hai,Yang, Fu-Ru,Huang, Meng,Zhou, Yan-Jun,Li, Ze-Jun,Wang, Xiu-Hui,Li, Guo-Xin,Jiang, Yi-Feng,Tong, Wu,Tong, Guang-Zhi.

[15]Molecular characterization of a novel reassortant H1N2 influenza virus containing genes from the 2009 pandemic human H1N1 virus in swine from eastern China. Peng, Xiuming,Wu, Haibo,Peng, Xiaorong,Cheng, Linfang,Jin, Changzhong,Xie, Tiansheng,Lu, Xiangyun,Wu, Nanping,Xu, Lihua.

[16]Immunohistochemical analysis of cell wall hydroxyproline-rich glycoproteins in the roots of resistant and susceptible wax gourd cultivars in response to Fusarium oxysporum f. sp Benincasae infection and fusaric acid treatment. Ma, Li,Xu, Chunxiang,Xie, Dasen,Samaj, Jozef. 2011

[17]Production and evaluation of a panel of monoclonal antibodies against Haemophilus paragallinarum. Zhang, P,Blackall, PJ,Yamaguchi, T,Iritani, Y. 2000

[18]Identification of a linear B-cell epitope on the avian leukosis virus P27 protein using monoclonal antibodies. Li, Xiaofei,Qi, Xiaole,Wang, Yongqiang,Gao, Honglei,Gao, Yulong,Wang, Xiaomei,Li, Xiaofei,Qin, Liting,Sun, Yingjun,Cui, Xuezhi,Gao, Yadong,Zhu, Haibo.

[19]Generation and characterization of monoclonal antibodies specific for 18 kDa antigen from Taenia solium cysticerci. Zhang, Shaohua,Luo, Xuenong,Guo, Aijiang,Zhu, Xueliang,Cai, Xuepeng.

[20]Development of a Rapid Immunochromatographic Lateral Flow Device Capable of Differentiating Phytase Expressed from Recombinant Aspergillus niger phyA2 and Genetically Modified Corn. Zhou, Xiaojin,Pu, Ling-Kui,Hui, Elizabeth,Zhang, Jun,Yu, Xiao-Lin,Tu, Zhiguan,Lin, Zhen,Zheng, Jian,Zhang, Jun,Liu, Oi,Liu, Oi,Zhane, Juan.

作者其他论文 更多>>