Mitochondria response to camptothecin and hydroxycamptothecine-induced apoptosis in Spodoptera exigua cells

文献类型： 外文期刊

第一作者： Zhang, Lan

作者： Zhang, Lan;Zhang, Yanning;Mao, Liangang;Jiang, Hongyun

作者机构：

关键词： Mitochondrion;Camptothecins;Mitochondrial membrane potential;Ca2+;Spodoptera exigua

期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：3.963； 五年影响因子：4.454 ）

ISSN： 0048-3575

年卷期： 2017 年 140 卷

页码：

收录情况： SCI

摘要： Camptothecin (CPT), a natural alkaloid extracted from Camptotheca acuminata Decne, exhibits potential insecticidal activities against various insect species. Our previous studies have showed that CPTs induced apoptosis in Spodoptera exigua Hubner cell line which is mediated preliminarily by the mitochondrial pathway. In this study, changes of mitochondrial morphologic and function were investigated to characterize mitochondrial responses in CPTs induced apoptosis. After incubation IOZCAS-Spex-II cells with CPT and HCPT, mitochondria exhibited obvious changes in the size, morphology and distribution, and ultrastructural alterations characterized by disruption of cristae and membrane. The typical characteristics of apoptosis, including chromatin condensation, nucleus shrivels, and cytoplasmic vacuoles were found. CPT and HCPT induced IOZCAS-Spex-II cell apoptosis accompanied with increased dramatically cytosolic Ca2+ and reduced mitochondrial membrane potential in the dose and time-dependent pattern. Cytochrome c release induced by CPT and HCPT was partially reduced in the presence of CsA, which suggested that the opening of mitochondrial permeability transition pore. Taken together, these results suggested the role of mitochondria in regulation of insect cell apoptosis, which provided the basic information for illustrating the apoptosis pathway in insects and for using reasonably CPTs to control insect pests. (C) 2017 Elsevier Inc. All rights reserved.

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