Evaluation of protective immunity induced by DNA vaccination with genes encoding Toxoplasma gondii GRA17 and GRA23 against acute toxoplasmosis in mice

文献类型： 外文期刊

第一作者： Zhu, Wei-Ning

作者： Zhu, Wei-Ning;Wang, Jin-Lei;Chen, Kai;Yue, Dong-Mei;Zhang, Xiao-Xuan;Huang, Si-Yang;Zhu, Xing-Quan;Zhu, Wei-Ning

作者机构：

关键词： Toxoplasma gondii;Toxoplasmosis;GRA17;GRA23;DNA vaccine;Protective immunity

期刊名称：EXPERIMENTAL PARASITOLOGY （ 影响因子：2.011； 五年影响因子：2.132 ）

ISSN： 0014-4894

年卷期： 2017 年 179 卷

页码：

收录情况： SCI

摘要： Toxoplasma gondii, an obligatory intracellular protozoan, can cause serious public health problems and economic losses worldwide. Two novel dense granule proteins (GRA17 and GRA23) were recently identified as T. gondii-secreted proteins which are localized to the parasitophorous vacuole membrane (PVM) and can mediate the movement of small molecules between the host cell and parasitophorous vacuole (PV). In the present study, we evaluated the protective immunity induced by DNA vaccination with genes encoding GRA17 and GRA23 against acute toxoplasmosis in mice. Eukaryotic expressing plasmids pVAX-TgGRA17 and pVAX-TgGRA23 were constructed. Then, BALB/c mice were intramuscularly immunized with pVAX-TgGRA17, pVAX-TgGRA23, or pVAX-TgGRA17 + pVAX-TgGRA23 followed by challenge infection with the highly virulent RH strain of T. gondii. The specific immune responses and protective efficacy against T. gondii were examined by cytokine and serum antibody measurements, lymphocyte proliferation assays, flow cytometry of lymphocytes and the survival time after challenge. Our results showed that mice immunized with pVAX-TgGRA17, pVAX-TgGRA23, or pVAX-TgGRA17 + pVAX-TgGRA23 induced specific humoral and cellular responses, with higher level of IgG antibody, increased levels of Th1-type cytokines IFN-gamma and IL-12 (p70), and CD3(+)CD4(+)CD8(-) and CD3(+)CD8(+)CD4(-) T cells, as well as prolonged survival time (9.1 +/- 0.32 days for pVAX-TgGRA17, 10.8 +/- 0.79 days for pVAX-TgGRA23, and 12.6 +/- 2.55 days for pVAX-TgGRA17 + pVAX-TgGRA23) compared to the blank control (7.11 +/- 033 days), PBS control (7.22 +/- 0.44 days), and pVAX I control (7.11 +/- 0.33 days). These results demonstrated that both TgGRA17 and TgGRA23 are potential vaccine candidates, TgGRA23 has a better immunogenicity, and co-immunization of pVAX-TgGRA17 and pVAX-TgGRA23 induces better protective efficacy. (C) 2017 Published by Elsevier Inc.

分类号：

  • 相关文献

[1]Protective efficacy of Toxoplasma gondii calcium-dependent protein kinase 1 (TgCDPK1) adjuvated with recombinant IL-15 and IL-21 against experimental toxoplasmosis in mice. Chen, Jia,Li, Zhong-Yuan,Huang, Si-Yang,Song, Hui-Qun,Zhou, Dong-Hui,Zhu, Xing-Quan,Chen, Jia,Zhu, Xing-Quan,Petersen, Eskild,Petersen, Eskild. 2014

[2]Protective immunity induced by a DNA vaccine expressing eIF4A of Toxoplasma gondii against acute toxoplasmosis in mice. Chen, Jia,Huang, Si-Yang,Li, Zhong-Yuan,Yuan, Zi-Guo,Zhou, Dong-Hui,Zhang, Nian-Zhang,Zhu, Xing-Quan,Chen, Jia,Li, Zhong-Yuan,Yuan, Zi-Guo,Petersen, Eskild,Petersen, Eskild,Zhu, Xing-Quan. 2013

[3]Synergy of mIL-21 and mIL-15 in enhancing DNA vaccine efficacy against acute and chronic Toxoplasma gondii infection in mice. Li, Zhong-Yuan,Chen, Jia,Zhou, Dong-Hui,Huang, Si-Yang,Song, Hui-Qun,Zhu, Xing-Quan,Li, Zhong-Yuan,Zhu, Xing-Quan,Petersen, Eskild.

[4]DNA vaccination with genes encoding Toxoplasma gondii antigens ROP5 and GRA15 induces protective immunity against toxoplasmosis in Kunming mice. Chen, Jia,Li, Zhong-Yuan,Huang, Si-Yang,Zhou, Dong-Hui,Zhu, Xing-Quan,Li, Zhong-Yuan,Zhu, Xing-Quan,Petersen, Eskild,Zhu, Xing-Quan. 2015

[5]DNA immunization with eukaryotic initiation factor-2 alpha of Toxoplasma gondii induces protective immunity against acute and chronic toxoplasmosis in mice. Chen, Jia,Huang, Si-Yang,Zhou, Dong-Hui,Li, Zhong-Yuan,Song, Hui-Qun,Zhu, Xing-Quan,Petersen, Eskild,Petersen, Eskild,Zhu, Xing-Quan. 2013

[6]Protective immunity against Toxoplasma gondii induced by DNA immunization with the gene encoding a novel vaccine candidate: calcium-dependent protein kinase 3. Zhang, Nian-Zhang,Huang, Si-Yang,Zhou, Dong-Hui,Chen, Jia,Zhu, Xing-Quan,Xu, Ying,Zhu, Xing-Quan,Tian, Wei-Peng,Lu, Jing,Zhu, Xing-Quan. 2013

[7]Immune responses and protection after DNA vaccination against Toxoplasma gondii calcium-dependent protein kinase 2 (TgCDPK2). Chen, Kai,Wang, Jin-Lei,Huang, Si-Yang,Yang, Wen-Bin,Zhu, Wei-Ning,Zhu, Xing-Quan,Huang, Si-Yang,Zhu, Xing-Quan,Yang, Wen-Bin,Zhu, Wei-Ning. 2017

[8]Co-administration of interleukins 7 and 15 with DNA vaccine improves protective immunity against Toxoplasma gondii. Chen, Jia,Li, Zhong-Yuan,Liu, Wen-Ge,Zhu, Xing-Quan,Chen, Jia,Petersen, Eskild,Zhu, Xing-Quan.

[9]Evaluation of immuno-efficacy of a novel DNA vaccine encoding Toxoplasma gondii rhoptry protein 38 (TgROP38) against chronic toxoplasmosis in a murine model. Xu, Ying,Zhang, Nian-Zhang,Tan, Qi-Dong,Chen, Jia,Lu, Jing,Zhu, Xing-Quan,Xu, Ying,Tan, Qi-Dong,Xu, Qian-Ming,Zhu, Xing-Quan. 2014

[10]Vaccination with a DNA vaccine encoding Toxoplasma gondii ROP54 induces protective immunity against toxoplasmosis in mice. Yang, Wen-Bin,Zhao, Guang-Hui,Yang, Wen-Bin,Zhou, Dong-Hui,Zou, Yang,Chen, Kai,Wang, Jin-Lei,Zhu, Xing-Quan,Liu, Qing,Zhu, Xing-Quan. 2017

[11]Evaluation of protective effect of pVAX-TgMIC13 plasmid against acute and chronic Toxoplasma gondii infection in a murine model. Yuan, Zi-Guo,Ren, Di,Zhou, Dong-Hui,Zhou, Yang,Zhu, Xing-Quan,Yuan, Zi-Guo,Ren, Di,Zhang, Xiu-Xiang,Li, Xiu-Zhen,Petersen, Eskild,Petersen, Eskild,Yang, Gui-Lian,Zhu, Xing-Quan. 2013

[12]A long-lasting protective immunity against chronic toxoplasmosis in mice induced by recombinant rhoptry proteins encapsulated in poly (lactide-co-glycolide) microparticles. Xu, Ying,Zhang, Nian-Zhang,Wang, Meng,Dong, Hu,Feng, Sheng-Yong,Guo, Hui-Chen,Zhu, Xing-Quan,Xu, Ying,Zhu, Xing-Quan.

[13]Protective efficacy of two novel DNA vaccines expressing Toxoplasma gondii rhomboid 4 and rhomboid 5 proteins against acute and chronic toxoplasmosis in mice. Zhang, Nian-Zhang,Xu, Ying,Wang, Meng,Chen, Jia,Huang, Si-Yang,Zhu, Xing-Quan,Xu, Ying,Petersen, Eskild,Huang, Si-Yang,Zhu, Xing-Quan. 2015

[14]DNA vaccination with a gene encoding VP60 elicited protective immunity against rabbit hemorrhagic disease virus. Yuan, Dongwei,Qu, Liandong,Liu, Jiasen,Guo, Dongchun,Jiang, Qian,Lin, Huan,Si, Changde.

[15]Toxoplasma gondii: Protective immunity induced by rhoptry protein 9 (TgROP9) against acute toxoplasmosis. Chen, Jia,Zhou, Dong-Hui,Li, Zhong-Yuan,Huang, Si-Yang,Song, Hui-Qun,Zhu, Xing-Quan,Petersen, Eskild,Petersen, Eskild,Li, Zhong-Yuan,Zhu, Xing-Quan. 2014

[16]Sequence variation in the Toxoplasma gondii ROP20 gene among strains from different hosts and geographical locations. Ning, H. R.,Zhu, X. Q.,Ning, H. R.,Wang, J. L.,Qin, S. Y.,Huang, S. Y.,Lou, Z. L.,Hu, L. Y.,Zhu, X. Q.,Qin, S. Y.,Lou, Z. L.,Huang, S. Y.,Zhu, X. Q.. 2015

[17]Characterization of mouse brain microRNAs after infection with cyst-forming Toxoplasma gondii. Xu, Min-Jun,Zhou, Dong-Hui,Huang, Si-Yang,Fan, Yi-Fan,Zhu, Xing-Quan,Nisbet, Alasdair J.,Zhu, Xing-Quan,Fan, Yi-Fan. 2013

[18]Sequence variation in Toxoplasma gondii MIC13 gene among isolates from different hosts and geographical locations. Ren, Di,Zhou, Yang,Lin, Ze-Ping,Lin, Rui-Qing,Wu, Song-Ming,Lin, Shao-Qi,Yuan, Zi-Guo,Ren, Di,Zhou, Dong-Hui,Xu, Min-Jun,Zhou, Yang,Wu, Song-Ming,Yuan, Zi-Guo,Yang, Jian-Fa,Zou, Feng-Cai. 2012

[19]Genetic characterization of Toxoplasma gondii from pigs from different localities in China by PCR-RFLP. Jiang, Hai-Hai,Huang, Si-Yang,Zhou, Dong-Hui,Zhang, Xiao-Xuan,Su, Chunlei,Zhu, Xing-Quan,Deng, Shun-Zhou,Su, Chunlei,Zhang, Xiao-Xuan. 2013

[20]Sequence Variation in Rhoptry Neck Protein 10 Gene among Toxoplasma gondii Isolates from Different Hosts and Geographical Locations. Zhao, Yu,Sun, Xiaolin,Zhao, Yu,Zhou, Donghui,Chen, Jia. 2017

作者其他论文 更多>>

微信小程序