Tauroursodeoxycholic acid enhances the development of porcine embryos derived from in vitro-matured oocytes and evaporatively dried spermatozoa

文献类型： 外文期刊

第一作者： Li, Xiao-Xia

作者： Li, Xiao-Xia;Diao, Yun-Fei;Wei, Hai-Jun;Wang, Shi-Yong;Cao, Xin-Yan;Zhang, Yu-Fei;Chang, Tong;Li, Dan-Li;Xu, Baozeng;Li, Xiao-Xia;Diao, Yun-Fei;Wei, Hai-Jun;Wang, Shi-Yong;Cao, Xin-Yan;Zhang, Yu-Fei;Chang, Tong;Li, Dan-Li;Xu, Baozeng;Li, Xiao-Xia;Kim, Min Kyu

作者机构：

期刊名称：SCIENTIFIC REPORTS （ 影响因子：4.379； 五年影响因子：5.133 ）

ISSN： 2045-2322

年卷期： 2017 年 7 卷

页码：

收录情况： SCI

摘要： Evaporative drying (ED) is an alternative technique for long-term preservation of mammalian sperm, which does not require liquid nitrogen or freeze-drying equipment, but offers advantages for storage and shipping at ambient temperature and low cost. However, the development of zygotes generated from these sperms was poor. Here, we demonstrated that the supplementation of tauroursodeoxycholic acid (TUDCA), an endogenous bile acid, during embryo culture improved the developmental competency of embryos derived from in vitro matured pig oocytes injected intracytoplasmically with boar ED spermatozoa by reducing the production of reactive oxygen species, the DNA degradation and fragmentation, and the expression of apoptosis-related gene Bax and Bak, and by increasing the transcription of anti-apoptosis gene Bcl-XL and Bcl-2. Furthermore, TUDCA treatment promoted the blastocyst quality manifested by the total cell numbers and the ratio of inner cell mass. Taken together, our data suggest that evaporative drying would be a potentially useful method for the routine preservation of boar sperm in combination with further optimization of subsequently embryo culture conditions.

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