Identification and expression analysis of miR-144-5p and miR-130b-5p in dairy cattle

文献类型： 外文期刊

第一作者： Li, Zhixiong

作者： Li, Zhixiong;Chen, Ling;Zhai, Mengxing;Chen, Si;Li, Na;Liu, Xiaolin;Li, Zhixiong;Wang, Hongliang

作者机构：

期刊名称：ARCHIVES ANIMAL BREEDING （ 影响因子：1.528； 五年影响因子：1.723 ）

ISSN： 0003-9438

年卷期： 2017 年 60 卷 3 期

页码：

收录情况： SCI

摘要： MicroRNAs (miRNAs) can coordinate the main pathways involved in innate and adaptive immune responses by regulating gene expression. To explore the resistance to mastitis in cows, miR-144-5p and miR-130b-5p were identified in bovine mammary gland tissue and 14 potential target genes belonging to the chemokine signaling pathway, the arginine and proline metabolism pathway and the mRNA surveillance pathway were predicted. Subsequently, we estimated the relative expression of miR-144-5p and miR-130b-5p in cow mammary tissues by using stem-loop quantitative real-time polymerase chain reaction. The results showed that the relative expression of miR-144-5p and miR-130b-5p in the mastitis-infected mammary tissues (n = 5) was significantly downregulated 0.14-fold (p < 0.01) and upregulated 3.34-fold (p < 0.01), respectively, compared to healthy tissues (n = 5). Our findings reveal that miR-144-5p and miR-130b-5p may have important roles in resistance to mastitis in dairy cattle.

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