Development of real-time and lateral flow dipstick recombinase polymerase amplification assays for rapid detection of goatpox virus and sheeppox virus

文献类型： 外文期刊

第一作者： Yang, Yang

作者： Yang, Yang;Qin, Xiaodong;Zhang, Xiangle;Zhao, Zhixun;Zhang, Wei;Zhu, Xueliang;Cong, Guozheng;Li, Yanmin;Zhang, Zhidong

作者机构：

关键词： Recombinase polymerase amplification;CaPV real-time RPA;CaPV RPA LFD;Goatpox virus;Sheeppox virus

期刊名称：VIROLOGY JOURNAL （ 影响因子：4.099； 五年影响因子：3.719 ）

ISSN： 1743-422X

年卷期： 2017 年 14 卷

页码：

收录情况： SCI

摘要： Background: Goatpox virus (GTPV) and sheeppox virus (SPPV), which belong to the Capripoxvirus (CaPV), are economically important pathogens of small ruminants. Therefore, a sensitive, specific and rapid diagnostic assay for detection of GTPV and SPPV is necessary to accurately and promptly control these diseases. Methods: Recombinase polymerase amplification (RPA) assays combined with a real-time fluorescent detection (real-time RPA assay) and lateral flow dipstick (RPA LFD assay) were developed targeting the CaPV G-protein-coupled chemokine receptor (GPCR) gene, respectively. Results: The sensitivity of both CaPV real-time RPA assay and CaPV RPA LFD assay were 3 x 102 copies per reaction within 20 min at 38 degrees C. Both assays were highly specific for CaPV, with no cross-reactions with peste des petits ruminants virus, foot-and-mouth disease virus and Orf virus. The evaluation of the performance of these two assays with clinical sample (n = 107) showed that the CaPV real-time RPA assay and CaPV RPA LFD assay were able to specially detect SPPV or GTPV present in samples of ovine in liver, lung, kidney, spleen, skin and blood. Conclusions: This study provided a highly time-efficient and simple alternative for rapid detection of GTPV and SPPV.

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