Identification of QTLs controlling grain protein concentration using a high-density SNP and SSR linkage map in barley (Hordeum vulgare L.)

文献类型: 外文期刊

第一作者: Fan, Chaofeng

作者: Fan, Chaofeng;Zhai, Huijie;Wang, Huifang;Yue, Yafei;Zhang, Minghu;Li, Jinghui;Wen, Shaozhe;Ni, Zhongfu;You, Mingshan;Fan, Chaofeng;Zhai, Huijie;Wang, Huifang;Yue, Yafei;Zhang, Minghu;Li, Jinghui;Wen, Shaozhe;Ni, Zhongfu;You, Mingshan;Guo, Ganggang;Zeng, Yawen

作者机构:

关键词: Barley (Hordeum vulgare L.);Grain protein concentration (GPC);QTL;SNP marker;Near isogenic line

期刊名称:BMC PLANT BIOLOGY ( 影响因子:4.215; 五年影响因子:4.96 )

ISSN: 1471-2229

年卷期: 2017 年 17 卷

页码:

收录情况: SCI

摘要: Background: Grain protein concentration (GPC) is a major determinant of quality in barley (Hordeum vulgare L.). Breeding barley cultivars with high GPC has practical value for feed and food properties. The aim of the present study was to identify quantitative trait loci (QTLs) for GPC that could be detected under multiple environments. Results: A population of 190 recombinant inbred lines (RILs) deriving from a cross between Chinese landrace ZGMLEL with high GPC (> 20%) and Australian cultivar Schooner was used for linkage and QTL analyses. The genetic linkage map spanned 2353.48 cM in length with an average locus interval of 2.33 cM. GPC was evaluated under six environments for the RIL population and the two parental lines. In total, six environmentally stable QTLs for GPC were detected on chromosomes 2H (1), 4H (1), 6H (1), and 7H (3) and the increasing alleles were derived from ZGMLEL. Notably, the three QTLs on chromosome 7H (QGpc. ZiSc-7H. 1, QGpc. ZiSc-7H. 2, and QGpc. ZiSc-7H. 3) that linked in coupling phase were firstly identified. Moreover, the genetic effects of stable QTLs on chromosomes 2H, 6H and 7H were validated using near isogenic lines (NILs). Conclusions: Collectively, the identified QTLs expanded our knowledge about the genetic basis of GPC in barley and could be selected to develop cultivars with high grain protein concentration.

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