Stereospecific recognition and rapid determination of d-amino acids in human serum based on luminescent metal-organic frameworks

文献类型： 外文期刊

第一作者： Liu, Qingju

作者： Liu, Qingju;Wang, Hui;Han, Ping;Tang, Saixue;Tang, Saixue

作者机构：

期刊名称：NEW JOURNAL OF CHEMISTRY （ 影响因子：3.925； 五年影响因子：3.629 ）

ISSN： 1144-0546

年卷期： 2022 年 46 卷 17 期

页码：

收录情况： SCI

摘要： The current luminescent metal-organic framework (MOF) based fluorescence detection mostly focuses on achiral molecules. The stereospecific recognition and determination of MOF-based detection remain challenging. A novel luminescent terbium-based MOF (Tb-MOF) based biosensing system with excellent performance for stereoselective detection of d-amino acids (d-AAs) was introduced in this paper. d-Amino acid oxidase catalyzes stereospecific oxidative deamination of d-AAs, producing H2O2. The generated H2O2 effectively quenches the fluorescence of Tb-MOF. Under optimum conditions, the d-AAs were detected with excellent efficiency. The linear working range was 0.5-100 mu M for d-Alanine (d-Ala) and 0. 5-5 mu M for d-Proline (d-Pro), respectively. The limit of detection (K-sv detection) for d-Ala and d-Pro was 0.09 mu M and 0.14 mu M, respectively. The detection can be performed within 30 min with high selectivity and long-term stability. The fluorescent sensing platform required simple fabrication and operation and was free from complicated reaction processes and expensive reagents. The present sensing system was successfully applied in d-AA detection in human serum with good recovery, indicating its potential application in detection in biological samples.

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