Gene cloning, expression, and characterization of trehalose-6-phosphate synthase from Pleurotus ostreatus

文献类型： 外文期刊

第一作者： Lei, Min

作者： Lei, Min;Wu, Xiangli;Zhang, Jinxia;Huang, Chenyang;Lei, Min;Wu, Xiangli;Zhang, Jinxia;Huang, Chenyang;Lei, Min;Wang, Hexiang

作者机构：

关键词： enzyme kinetics;heat treatment;Pleurotus ostreatus;TPS1;trehalose

期刊名称：JOURNAL OF BASIC MICROBIOLOGY （ 影响因子：2.281； 五年影响因子：2.516 ）

ISSN： 0233-111X

年卷期： 2017 年 57 卷 7 期

页码：

收录情况： SCI

摘要： Trehalose-6-phosphate synthase (TPS; EC2.4.1.15) catalyzes the first step in trehalose synthesis, which involves transfer of glucose from uridine diphosphate glucose (UDPG) to glucose 6-phosphate (G6P) to form trehalose-6-phosphate. To determine the gene and enzymatic characteristics of TPS in Pleurotus ostreatus, we cloned and sequenced the cDNA of PoTPS1, which contains a 1665 bp open reading frame that encodes a 554-amino acid protein with a predicted molecular weight of 62.01 kDa. This gene was expressed in Escherichia coli BL21 and then the recombinant protein was purified and characterized. Results showed that the optimum pH and temperature for the recombinant PoTPS1 were 7.4 and 30 degrees C, respectively; the Km value against G6P and UDPG were 0.14 and 0.17 mM, respectively, and the V-max and K-cat values were 91.86 nkat/g and 5.89 s(-1), respectively. Trehalose content was as high as 158.88 mg g(-1) dry weight after heat treatment at 40 degrees C for 15 h, which was consistent with highest TPS1 activity at that time point. This result indicated that PoTPS1 was responsible for trehalose synthesis in P. ostreatus.

分类号：

  • 相关文献

[1]Carbohydrate changes during growth and fruiting in Pleurotus ostreatus. Zhou, Shuai,Ma, Fuying,Zhang, Xiaoyu,Zhou, Shuai,Zhang, Jingsong.

[2]Molecular analysis of caffeoyl residues related to pigmentation in green cotton fibers. Hongjie Feng,Yonglin Yang,Shichao Sun,Sun, Jie,Zhu, Heqin,Yanjun Li,Lin Zhang,Jingkui Tian,Qianhao Zhu,Zili Feng,Heqin Zhu,Jie Sun.

[3]Characterization of a root-specific beta-thioglucoside glucohydrolase gene in Carica papaya and its recombinant protein expressed in Pichia pastoris. Wang, Meng,Li, Dingqin,Sun, Xuepiao,Nong, Han,Zhang, Jiaming,Zhu, Yun Judy,Nong, Han. 2009

[4]Mutations in two amino acids in phyI1s from Aspergillus niger 113 improve its phytase activity. Tian, Yong-Sheng,Peng, Ri-He,Xu, Jing,Zhao, Wei,Gao, Feng,Fu, Xiao-Yan,Xiong, Ai-Sheng,Yao, Quan-Hong.

[5]Extraction of Oil from Flaxseed (Linum usitatissimum L.) Using Enzyme-Assisted Three-Phase Partitioning. Tan, Zhi-jian,Yi, Yong-jian,Wang, Hong-ying,Zhou, Wan-lai,Wang, Chao-yun,Yang, Zi-zhen,Li, Fen-fang.

[6]Identification and characterization of a cathepsin-L-like peptidase in Eimeria tenella. Liu, Renqiang,Liu, Aijun,Wang, Ming,Ma, Xueting,Cai, Jianping,Zhang, Lei,Wang, Ming.

[7]Invertebrate Trehalose-6-Phosphate Synthase Gene:Genetic Architecture, Biochemistry, Physiological Function, and Potential Applications. Tang, Bin,Wang, Shi-Gui,Wang, Hui-Juan,Tang, Bin,Cui, Shuai-Ying,Wang, Su,Zhang, Jia-Yong. 2018

[8]Influence of Photoperiod on the Accumulation of Metabolites during Diapause Induction in Cotesia vestalis (Haliday) (Hymenoptera: Braconidae). Hao, Zhong-Ping,Chen, Chao,Shi, Zu-Hua,Hao, Zhong-Ping.

[9]Gene cloning, expression, and characterization of a novel trehalose synthase from Arthrobacter aurescens. Wu Xiuli,Ding Hongbiao,Yue Ming,Qiao Yu.

[10]Determination of Carbohydrates by High Performance Anion Chromatography-Pulsed Amperometric Detection in Mushrooms. Tang, Qing-Jiu. 2012

[11]Induction of mucosal immune responses and protection of cattle against direct-contact challenge by intranasal delivery with foot-and-mouth disease virus antigen mediated by nanoparticles. Pan, Li,Zhang, Zhongwang,Lv, Jianliang,Zhou, Peng,Hu, Wenfa,Fang, Yuzhen,Chen, Haotai,Liu, Xinsheng,Shao, Junjun,Zhao, Furong,Ding, Yaozhong,Lin, Tong,Chang, Huiyun,Zhang, Jie,Zhang, Yongguang,Wang, Yonglu,Zhang, Zhongwang,Lv, Jianliang,Zhou, Peng,Hu, Wenfa,Fang, Yuzhen,Chen, Haotai,Liu, Xinsheng,Shao, Junjun,Zhao, Furong,Ding, Yaozhong,Lin, Tong,Chang, Huiyun,Zhang, Jie,Zhang, Yongguang,Wang, Yonglu. 2014

[12]Cloning, expression and characterization of a trehalose synthase gene from rhodococcus opacus. Yan, Junyan,Qiao, Yu,Ding, Hongbiao,Hu, Jun,Ding, Hongbiao.

[13]Effects of starvation on the carbohydrate metabolism in Harmonia axyridis (Pallas). Shi, Zuo-Kun,Wang, Shi-Gui,Zhang, Lu,Xu, Yan-Xia,Tang, Bin,Wang, Su,Guo, Xiao-Jun,Zhang, Fan. 2017

[14]Two novel soluble trehalase genes cloned from Harmonia axyridis and regulation of the enzyme in a rapid changing temperature. Shi, Zuokun,Liu, Xiaojun,Xu, Qingye,Qin, Zi,Wang, Shigui,Tang, Bin,Shi, Zuokun,Wang, Su,Zhang, Fan.

[15]Trehalase in Harmonia axyridis (Coleoptera: Coccinellidae): effects on beetle locomotory activity and the correlation with trehalose metabolism under starvation conditions. Tang, Bin,Qin, Zi,Wang, Su,Guo, Xiao-Jun,Zhang, Fan,Tang, Bin,Shi, Zuo-Kun,Wang, Shi-Gui.

[16]Physiological roles of trehalose in Leptinotarsa larvae revealed by RNA interference of trehalose-6-phosphate synthase and trehalase genes. Shi, Ji-Feng,Xu, Qing-Yu,Sun, Qiang-Kun,Meng, Qing-Wei,Mu, Li-Li,Li, Guo-Qing,Guo, Wen-Chao.

[17]The use of Crofton Weed (Eupatorium adenophorum Spreng) Biomass in Mushroom Cultivation. Ya Tang,Liu Benhong,Qiu Dunlian. 2009

[18]Cloning and characterization of the pyrG gene of Pleurotus ostreatus and Pleurotus eryngii. Yin, Yonggang,Li, Ming,Yin, Yonggang,Liu, Yu,Wang, Shouxian,Niu, Liying,Zhao, Shuang,Geng, Xiaoli,Xu, Feng,Li, Huamin. 2012

[19]Establishment of an efficient transformation system for Pleurotus ostreatus. Lei, Min,Wu, Xiangli,Zhang, Jinxia,Huang, Chenyang,Lei, Min,Wang, Hexiang. 2017

[20]Isolation of DNA topoisomerase II gene from Pleurotus ostreatus and its application in phylogenetic analysis. Li, C. X.,Zhang, J. X.,Huang, C. Y.,Chen, Q.,Wang, H. X..

作者其他论文 更多>>

微信小程序