Molecular identification of Trichogramma species from South and South-East Asia and natural Wolbachia infection

文献类型： 外文期刊

第一作者： Hou, Mao-Lin

作者： Hou, Mao-Lin;Song, Kai

作者机构：

期刊名称：ENTOMOLOGICA FENNICA （ 影响因子：0.767； 五年影响因子：0.755 ）

ISSN： 0785-8760

年卷期： 2017 年 28 卷 2 期

页码：

收录情况： SCI

摘要： Trichogramma wasps were collected from the parasitized eggs of lepidopteran pests from 21 sampling sites in East Asia and South-East Asia. Six Trichogramma species were identified based on the molecular identification method using the internal transcribed spacer 2 (ITS2) region of the rDNA of Trichogramma chilonis, T evanescens, T ostriniae, T embryophagum, T dendrolimi and T japonicum. The results of molecular identification were confirmed by morphological identification. Additionally, natural populations were screened for the prevalence of Wolbachia. Five out of 21 populations were infected by the same Wolbachia strain, which was identified by using Wolbachia wsp gene and multi locus sequencing approach. The phylogenetic analysis of Wolbachia wsp sequences revealed that the Wolbachia strain was classified in the strain wEvaA in the group of EvA of the supergroup A.

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