Microdissection of additional chromosome in common wheat-Th. intermedium TAI-27 and screening of its special probe

文献类型： 外文期刊

第一作者： Tian, C

作者： Tian, C;Lu, YF;Deng, JX;Li, B;Zhang, XY;Liu, GT

作者机构：

关键词： Th. intermedium;chromosome;microdissection;special probe

期刊名称：SCIENCE IN CHINA SERIES C-LIFE SCIENCES （ 影响因子：1.61； 五年影响因子：1.148 ）

ISSN： 1006-9305

年卷期： 2000 年 43 卷 1 期

页码：

收录情况： SCI

摘要： It was identified that there were 4 St chromosomes derived from Th. intermedium in common wheat-Th. intermedium alien additional line TAI-27 by in situ hybridization, Two St chromosomes added to wheat chromosome in TAI-27 as well as two of them replaced two of 42 in wheat chromosomes. This indicates that TAI-27 is not only an alien additional line, but also a replacing line. The additional chromosomes in TAI-27 were microdissected and a microcloning library was constructed. A special probe of Th. intermedium was obtained from a microcloning library. The sequence analysis indicated that there were no homology with Genebank data. This makes it possible to screen genes with the disease-resistance, adversity-tolerance and fine character from Th.infermedium.

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