Germination of somatic embryos and plant recovery in cotton (Gossypium hirsutum L.)
文献类型: 外文期刊
第一作者: Liu, F
作者: Liu, F;Wang, QL
作者机构:
关键词: Gossypium hirsutum L.;somatic embryo;plant recovery
期刊名称:PHYTON-INTERNATIONAL JOURNAL OF EXPERIMENTAL BOTANY ( 影响因子:1.039; 五年影响因子:0.77 )
ISSN: 0031-9457
年卷期: 2000 年 68 卷
页码:
收录情况: SCI
摘要: Plant recovery from somatic embryos and the response to basal medium, hormones and sucrose were studied in cotton (Gossypium hirsutum L.). Only late torpedo and cotyledonary stage embryos were able to germinate and develop into whole plants. BT basal medium promoted root development, MS medium promoted shoot development and SH basal medium promoted the balanced growth of roots and shoots. KT was disadvantageous to germination and plant recovery. The percentages of embryos forming shoots and whole plants were increased to 83.3% and 50% by adding 0.1 mg/L NAA, but NAA concentrations (higher than 0.1 mg/L) inhibited embryo germination and plant regeneration. The best concentration of sucrose for plant formation from cotton somatic embryos was 20 g/L.
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