In planta soybean transformation technologies developed in China: Procedure, confirmation and field performance

文献类型： 外文期刊

第一作者： Hu, CY

作者： Hu, CY;Wang, LZ

作者机构：

关键词： glycine max;soybean;genetic transformation;pollen-tube-pathway;in planta;non-in vitro culture

期刊名称：IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT （ 影响因子：2.252； 五年影响因子：2.139 ）

ISSN： 1054-5476

年卷期： 1999 年 35 卷 5 期

页码：

收录情况： SCI

摘要： Soybean is a recalcitrant species for in vitro manipulation. Chinese scientists developed two in planta non-tissue culture soybean transformation procedures: (1) via the "pollen-tube-pathway" to introduce exogenous genomic total DNA of Glycine gracilis, the seed of which consisted of 50% protein, and (2) "ovarian injection" with exogenous plasmid DNA containing atrazine-resistant gene. A high yield and high seed protein (45.44%) cultivar, 'Heisheng 101', resulted from the first method, and atrazine-resistant F-1, F-2, and F-3 plants were obtained from the second method. Both exogenous single-genic Mendelian traits anti multi-genic quantitative traits were transferrable with these simple and inexpensive procedures. However, some controversy exists with the acceptance of these novo procedures; mainly because instead of the standard Southern blotting, the RAPD and dot blotting techniques were used in the molecular confirmation of the transgenic status in the reported studies.

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