Detection of the rye chromatin in multispikelet wheat germplasm 10-A background using fluorescence in situ hybridization (FISH) and RFLP markers

文献类型: 外文期刊

第一作者: Wei, YM

作者: Wei, YM;Zheng, YL;Zhou, RH;Jia, JZ

作者机构:

关键词: wheat;rye;multispikelet wheat;1RS/1BL translocation;gliadin;fluorescence in situ hybridization;RFLP

期刊名称:ACTA BOTANICA SINICA ( 影响因子:0.599; )

ISSN: 0577-7496

年卷期: 1999 年 41 卷 7 期

页码:

收录情况: SCI

摘要: The multispikelet genes of lye (Secale cereale L,.) in common wheat (Triticum, aestivum. L.) line 10-A were detected by APAGE, fluorescence in situ hybridization (FISH) and RFLP markers. APAGE analysis indicated that the 10-A possesed the gliadin marker Gld1B3 of 1RS. Using fluorescence labeled total genomic DNA of rye as probes and common wheat genomic DNA for blocking, in situ hybridization showed that 1RS of rye was transferred to multispikelet wheat line 10-A. The restriction fragments located on the short arm of chromosome 1B were missing and the restriction fragments of IRS were present when the probes, which have been identified on the short arm of the homologous group 1, were used in RFLP analysis. These results suggested that the multispikelet wheat line 10-A carried the 1RS/1BL wheat-rye translocation chromosome. The utilization of 10-A in wheat improvement was discussed.

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