Construction of vector with IMT1 and its gene expression in transgenic tobacco leaf cells associated with salt tolerance

文献类型： 外文期刊

第一作者： Dong, YZ

作者： Dong, YZ

作者机构：

关键词： Imt1;construction of vector;Nicotiana tabacum;gene expression;salt tolerance

期刊名称：ACTA BOTANICA SINICA （ 影响因子：0.599； ）

ISSN： 0577-7496

年卷期： 1999 年 41 卷 2 期

页码：

收录情况： SCI

摘要： The plant expression vector with Imt1 (inositol O-methyltransferase) gene, pDH5, was constructed. The Imt1 gene was introduced to tobacco (Nicotiana tabacum cv. SR1) via Agrobacterium-mediated transformation. Thirty kanamycin-resistant shoots in MSS (MSO + 1 mg/L 6-BA + 30 g/L sucrose) selective medium was transferred to MSr (MSO + 3 g/L sucrose + 7 g/L glucose) medium supplemented with 1.2% similar to 1.5% NaCl for rooting. Eight plantlets rooted among the thirty shoots. PCR and Southern blot assay indicated that the Imt1 gene was integrated into tobacco genome in six plantlets. HPLC analysis showed that the product of Imt1 gene, D-ononitol accumulated in tobacco fresh leaf cells in a scope of 100 similar to 654 nmol/g FW. Western blot demonstrated the expression of Imt1 gene in tobacco cells.

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