Nodulation and molecular characterization of pure cultures isolated from root nodules of Coriaria nepalensis

文献类型： 外文期刊

第一作者： Hu, CJ

作者： Hu, CJ;Zhou, PZ;Zhou, Q;Chen, HK;Akkermans, ADL

作者机构：

关键词： Frankia;Coriaria nepalensis;nodulation;molecular characterization

期刊名称：CHINESE SCIENCE BULLETIN （ 影响因子：1.649； 五年影响因子：1.738 ）

ISSN： 1001-6538

年卷期： 1998 年 43 卷 8 期

页码：

收录情况： SCI

摘要： Four strains with typical morphology of actinomycete genus of Frankia were isolated from root nodules of Coriaria nepalensis. They were shown to nodulate the seedlings of host plant and hybridize with Frankia 16S rRNA targeted oligonucleotide probes, indicating that they did belong to the genus Frankia. Furthermore, by nifHDK probe hybridizations, the homologous fragments of nifHDK genes were detected among the bacteria, and they were located in various sizes of restriction fragments of total DNA, showing diverse patterns of restriction fragment length polymorphisms of nifHDK gene (nifHDK-RFLPs). The PCR-based amplification and cloning of nifH gene throw light on the molecular phylogeny of Coriaria-infective Frankia.

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