文献类型： 外文期刊

第一作者： Jia, MH

作者： Jia, MH

作者机构：

期刊名称：SEEKING AGRICULTURAL PRODUCE FREE OF PESTICIDE RESIDUES

ISSN：

年卷期： 1998 年 85 期

页码：

收录情况： SCI

摘要： An immunoassay (IA) was developed for monitoring the sulfonylurea herbicide metsulfuron-methyl (MS) residues. Hapten-protein conjugates were prepared by covalently linking 2-methoxycarbonyl phenylsulfonamide (2-MPS) moiety of metsulfuron-methyl through its amino group to proteins with an additional succinic acid spacer arm. Antibodies with high affinity and specificity against MS were yielded by hapten-BSA (bovine serum albumin) conjugate (MSH-BSA) as an immunogen coupled by using active ester method (AE). Most of other MS analogues and degradation products showed almost no cross-reactivity with the antibodies. Several other sulfonylureas with the corresponding 2-MPS moiety of MS could be recognised by the antibodies. This indicates the antibodies can also be used as essential reagent to develop other IAs for determinations of a group of sulfonylurea herbicides with the same moiety besides MS. The competitive ELISA based on immobilised hapten-protein conjugate format were established and proved available to determine free MS in aqueous media. The IC50 of ng/mL level were achieved in this formatted assay. The effects of pH, ionic strength and sample matrix on the ELISAs were investigated for assay optimisation. The fortified experiments provided acceptable recoveries and detection limitations. The results suggested the ELISAs could be used as an alternate and promising approach to analysis for the herbicide residue in water and soil. Furthermore. the potential progress which would be made in China for studies and application of IAs to monitoring sulfonylurea herbicide residue were introduced briefly. A hybrid methodology referred to immunoaffinity column combined with conventional methods will also be discussed as a promising and potential approach to sulfonylurea residual monitoring.

分类号：