Transgenic fertile japonica rice plants expressing a modified crylA(b) gene resistant to yellow stem borer

文献类型： 外文期刊

第一作者： Wu, C

作者： Wu, C;Fan, Y;Zhang, C;Oliva, N;Datta, SK

作者机构：

关键词： transgenic rice;particle bombardment;crylA(b);yellow stem borer resistance

期刊名称：PLANT CELL REPORTS （ 影响因子：4.57； 五年影响因子：4.463 ）

ISSN： 0721-7714

年卷期： 1997 年 17 卷 2 期

页码：

收录情况： SCI

摘要： The japonica rice variety Taipei 309 was co-transformed by particle bombardment of immature embryo-derived embryogenic calli with a modified delta-endotoxin gene cryIA(b) of Bacillus thuringiensis (Bt) under the control of the rice Actin1 promoter, and the hygromycin resistance gene, hph driven by the CaMV35S promoter. Selected transgenic rice plants showed enhanced insecticidal activity against yellow stem borer (Scirpophaga incertulas), with mortality rates reaching up to 100% in a bioassay with cut stems. Introduction and expression of the Actin1 promoter-Bt gene into rice provides japonica rice germplasm resistant to insect attack.

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