Quality of the entomopathogenic nematode Steinernema carpocapsae produced on different media

文献类型： 外文期刊

第一作者： Yang, HW

作者： Yang, HW;Jian, H;Zhang, SG;Zhang, GY

作者机构：

关键词： entomopathogenic nematode;artificial culture;nematode quality;quality control

期刊名称：BIOLOGICAL CONTROL （ 影响因子：3.687； 五年影响因子：3.962 ）

ISSN： 1049-9644

年卷期： 1997 年 10 卷 3 期

页码：

收录情况： SCI

摘要： The characteristics of morphometric (body length and width), biochemical (fatty acid content), motility, and penetration rate of infective juveniles of Steinernema carpocapsae Beijing strain reared on four different culture media [e.g., plant protein medium (I), animal protein medium (II), plant and animal protein medium (III), and in vivo culture (TV) were systematically compared in this research. The results showed that the average lengths of infective juveniles were 497.4 +/- 0.09, 514.3 +/- 0.08, 525.7 +/- 0.09, and 556.6 +/- 0.09 mu m, the average widths were 24.9 +/- 0.006, 25.6 +/- 0.005, 26.1 +/- 0.006, and 27.9 +/- 0.004 mu m, and the average dry weights per million infective juveniles were 49.2 a 2.2, 58.6 +/- 2.4, 59.6 +/- 1.8, and 80.7 +/- 1.7 mg cultured by media I, II, III, and TV, respectively. The highest relative content of fatty acid of infective juveniles was obtained from medium TV at 15.4 +/- 1.2 x 10(5) mu V/s, and the lowest one was 6.76 +/- 0.3 x 10(5) mu V/s from medium I and 11.8 +/- 0.2 x 10(5) and 13.7 +/- 0.3 x 10(5) mu V/s from media II and III, respectively. The numbers of nematodes that moved a vertical distance of 5 cm in sand column within 48 h were 24 +/- 3.6, 75 +/- 11.6, 69 +/- 9.7, and 92 +/- 13.2 and the penetration rates into the Galleria larva within 24 h were 2.8 +/- 0.45, 6.0 +/- 1.14, 6.4 +/- 0.74, and 6.0 +/- 0.7% from media I to IV, respectively. The results indicated that the quality of entomopathogenic nematode was influenced by the cultural medium component. The animal protein, especially from insects which were presented in media II, III, and TV, has a strong positive effect on nematode quality. (C) 1997 Academic Press.

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