Attempted transmission of Babesia major by Boophilus microplus

文献类型: 外文期刊

第一作者: Yin, H

作者: Yin, H;Lu, WS;Zhang, QC;Lu, WX;Luo, JX;Dou, HF

作者机构:

期刊名称:TROPICAL ANIMAL HEALTH AND PRODUCTION ( 影响因子:1.559; 五年影响因子:1.681 )

ISSN: 0049-4747

年卷期: 1997 年 29 卷 4 期

页码:

收录情况: SCI

摘要: Two experiments were carried out to determine if Babesia major could be transmitted by Boophilus microplus. In experiment 1, a Babesia-free batch of laboratory reared Bo. microplus larvae were applied to an intact calf infected by inoculation with a B. major stabilate. The calf showed a B. major parasitaemia while the larvae, nymphs and adult ticks were engorging. The engorged females were cultured and batches were incubated at one of the three following temperatures: 24, 28 or 32 degrees C. Approximately 10,000 larvae derived from each of the females were used to infest each of three splenectomized calves. In experiment 2, Babesia-free Bo. microplus larvae were applied to a splenectomized calf; the calf was injected with B. major stabilate and showed a B. major parasitaemia during the adult stage of tick development. The engorged females were incubated at room temperature and the resulting larvae (approximately 10,000) were used to infest a splenectomized calf. Examination of blood films for the presence of B. major from the four calves infested by the second generation larvae in the two experiments were negative.

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