RAPD polymorphisms in Aegilops geniculata Roth (Ae-ovata auct non L)

文献类型： 外文期刊

第一作者： Zhang, XY

作者： Zhang, XY;Wang, RRC;Dong, YS

作者机构：

关键词： Aegilops geniculata;RAPD;polymorphism;gene bank

期刊名称：GENETIC RESOURCES AND CROP EVOLUTION （ 影响因子：1.524； 五年影响因子：1.713 ）

ISSN： 0925-9864

年卷期： 1996 年 43 卷 5 期

页码：

收录情况： SCI

摘要： Genetic diversity of eighteen accessions of Ae. geniculata (2n = 28; UUMM) was assessed using the random amplified polymorphic DNA (RAPD) technique. We optimized RAPD conditions including the template DNA, the concentration of AmpliTaq DNA polymerase Stoffel fragment, and MgCl2 concentration for revealing polymorphisms. Thirty-eight decamer oligonucleotides were individually used as primers under optimized conditions. Seventeen of these primers produced polymorphic RAPDs among the 18 accessions of Ae. geniculata. Polymorphisms were recorded by noting presence or absence of an amplification product from the total genomic DNA. Comparisons of unique and shared amplification products of each pair of accessions were used to generate genetic similarity coefficients (GSCs). These GSCs were used to construct a phenogram using an unweighted pair-group method with arithmetical averages (UPGMA). The phenogram shows that RAPD data is useful in the measurement of genetic variation or similarity within a species. It also indicates that we can select eight or nine accessions of the eighteen accessions to maintain at least 80% genetic variability of the Chinese collection of Ae. geniculata.

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