PLANT-REGENERATION FROM RICE (ORYZA-SATIVA L.) EMBRYOGENIC SUSPENSION CELLS CRYOPRESERVED BY VITRIFICATION

文献类型： 外文期刊

第一作者： HUANG, CN

作者： HUANG, CN;WANG, JH;YAN, QS;ZHANG, XQ;YAN, QF

作者机构：

关键词： CRYOPRESERVATION;VITRIFICATION;CELL SUSPENSION;ORYZA SATIVA

期刊名称：PLANT CELL REPORTS （ 影响因子：4.57； 五年影响因子：4.463 ）

ISSN： 0721-7714

年卷期： 1995 年 14 卷 11 期

页码：

收录情况： SCI

摘要： Rice cells were precultured for 10 d in medium containing 60 g/L sucrose and subsequently for 1 d in medium supplemented with 0. 4 M sorbitol. After loading with 25%PVS2 at 22 degrees C for 10 min and dehydration in 100%PVS2 at 0 degrees C for 7. 5 min, they were plunged into liquid nitrogen directly. Survival was 45. 0 +/- 5. 1% (based on the reduction of triphenyl tetrazolium chloride) following warming and unloading. For regrowth, cells were plated on semi-solid medium replenished with 40% (w/v) starch for 2d prior to reculture. Cell suspensions were reestablished and plants were regenerated from recovered cells. Twenty eight plants set seeds in the greenhouse.

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