文献类型: 外文期刊
第一作者: QIAN, HR
作者: QIAN, HR;ZHUANG, JY;LIN, HX;LU, J;ZHENG, KL
作者机构:
关键词: RESTRICTION FRAGMENT LENGTH POLYMORPHISM (RFLP);PROBE SCREENING;SUBSPECIES DIFFERENTIATION;WIDE COMPATIBILITY VARIETY;ORYZA SATIVA L
期刊名称:THEORETICAL AND APPLIED GENETICS ( 影响因子:5.699; 五年影响因子:5.565 )
ISSN: 0040-5752
年卷期: 1995 年 90 卷 6 期
页码:
收录情况: SCI
摘要: Sixty-eight indica-japonica tester-differentiating RFLP probes were tested in seven indica and seven japonica varieties of rice (Orysa sativa L.) with four enzyme digestions (EcoRI, EcoRV, HindIII and DraI). Twenty-one DNA clones were isolated as indica-japonica subspecies-differentiating probes. A set of 13 probes was established as core probes for subspecies differentiation and a pooled blotting analysis was carried out to facilitate the application of RFLP in rice genetics and breeding practice. A dendrogram of 12 wide-compatibility varieties was constructed based on RFLPs detected by 13 core probes with single enzyme digestions. It was speculated that most RFLPs of indica-japonica differentiating probes were generated by insertions/deletions, which may be of great significance for the origin and differentiation of subspecies in Oryza sativa L.
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