ATP-Citrate Lyase Gene (SoACLA-1), a Novel ACLA Gene in Sugarcane, and Its Overexpression Enhance Drought Tolerance of Transgenic Tobacco

文献类型： 外文期刊

第一作者： Li, Jian

作者： Li, Jian;Sun, Bo;Liu, Jia-Yi;Zhao, Wen-Hui;Huang, Chan;Yang, Li-Tao;Li, Yang-Rui;Yang, Li-Tao;Li, Yang-Rui

作者机构：

关键词： Sugarcane;ATP-citrate lyase;Overexpression;Drought stress;Response tolerance;Tobacco

期刊名称：SUGAR TECH （ 影响因子：1.591； 五年影响因子：1.688 ）

ISSN： 0972-1525

年卷期： 2017 年 19 卷 3 期

页码：

收录情况： SCI

摘要： ATP-citrate lyase catalyzes cracking citrate to produce acetyl CoA in the cytoplasm and plays an important role in normal growth and development of plants. In this study, SoACLA-1 gene in sugarcane was cloned and characterized. The amino acid sequence of SoACLA-1 showed high homology with other ACLAs families, and they were clustered together in the phylogenetic tree. On transcript level, SoACLA-1 expressed strongly in root and stalk and responded to polyethylene glycol 6000, drought and ABA stresses. The results indicated that SoACLA-1 possibly involves in stress-responsive metabolic process. PCR analysis was performed to confirm the putative transgenic type in T1 tobacco plants. Overexpression of SoACLA-1 enhanced the tolerance of tobacco plants to drought. The sense transgenic tobacco plants were healthier, had longer roots and higher contents of relative water content and chlorophyll and activities of superoxide dismutase, peroxidase and catalase, but lower ion leakage and contents of malondialdehyde (MDA) and H2O2 than the wild-type and antisense transgenic plants under drought stress. In conclusion, SoACLA-1 plays an important role as a positive factor in drought stress response pathways and can be utilized for transformation to improve crop drought tolerance.

分类号：

  • 相关文献

[1]Overexpression of sugarcane gene SoSnRK2.1 confers drought tolerance in transgenic tobacco. Sun, Bo,Niu, Jun-Qi,Tan, Qin-Liang,Li, Jian,Yang, Li-Tao,Li, Yang-Rui,Yang, Li-Tao,Li, Yang-Rui.

[2]Correlation between AS1 gene expression and seed protein contents in different soybean (Glycine max [L.] merr.) cultivars. Wan, TF,Shao, GH,Shan, XC,Zeng, NY,Lam, HM.

[3]A wheat R2R3-MYB gene, TaMYB30-B, improves drought stress tolerance in transgenic Arabidopsis. Zhang, Lichao,Zhao, Guangyao,Xia, Chuan,Jia, Jizeng,Liu, Xu,Kong, Xiuying. 2012

[4]A Cotton MYB Transcription Factor, GbMYB5, is Positively Involved in Plant Adaptive Response to Drought Stress. Chen, Tianzi,Li, Wenjuan,Liu, Aimin,Zhang, Baolong,Li, Wenjuan,Hu, Xuehong,Guo, Jiaru,Liu, Aimin.

[5]Overexpression of cotton (Gossypium hirsutum) dirigent1 gene enhances lignification that blocks the spread of Verticillium dahliae. Haiyan Shi,Zhihao Liu,Li Zhu,Chaojun Zhang,Yun Chen,Ying Zhou,Fuguang Li,Xuebao Li. 2012

[6]Molecular Cloning and Function Analysis of Two SQUAMOSA-Like MADS-Box Genes From Gossypium hirsutum L.. Wenxiang Zhang,Shuli Fan,Chaoyou Pang,Hengling Wei,Jianhui Ma,Meizhen Song,Shuxun Yu. 2013

[7]Virus-Based MicroRNA Silencing and Overexpressing in Common Wheat (Triticum aestivum L.). Jian, Chao,Chi, Qing,Wang, Shijuan,Ma, Meng,Liu, Xiangli,Zhao, Huixian,Han, Ran,Zhao, Huixian. 2017

[8]Isolation and characterization of a stress responsive small GTP-binding protein AhRabG3b in peanut (Arachis hypogaea L.). Yu, Shan-Lin,Sui, Jiong-Ming,Li, Rui,Fan, Qian-Cheng,Zheng, Chun-Hua,Wang, Jing-Shan,Qiao, Li-Xian,Song, Lin. 2013

[9]Expression regulation of a xylanase inhibitor gene riceXIP in rice (Oryza sativa L.). Zhan, Yihua,Sun, Xiangyu,Xu, Ying,Huang, Yingying,Jiang, Dean,Weng, Xiaoyan,Sun, Renjie,Hou, Chunxiao,Hou, Chunxiao. 2017

[10]Effect of the expression and knockdown of citrate synthase gene on carbon flux during triacylglycerol biosynthesis by green algae Chlamydomonas reinhardtii. Cai, Jiajia,Fei, Xiaowen,Deng, Xiaodong. 2013

[11]Characterization of a pathway-specific activator of milbemycin biosynthesis and improved milbemycin production by its overexpression in Streptomyces bingchenggensis. He, Hairong,Liu, Hui,Wang, Haiyan,Xiang, Wensheng,He, Hairong,Liu, Hui,Wang, Xiangjing,Xiang, Wensheng. 2016

[12]Cloning, Expression, and Characterization of a New Streptomyces sp S27 Xylanase for Which Xylobiose is the Main Hydrolysis Product. Li, Ning,Shi, Pengjun,Yang, Peilong,Wang, Yaru,Luo, Huiying,Bai, Yingguo,Zhou, Zhigang,Yao, Bin. 2009

[13]Expression and functional characterization of a gene associated with retinoid-interferon-induced mortality 19 (GRIM-19) from orange-spotted grouper (Epinephelus coioides). Shi, Yan,Zhu, Xinping,Chen, Kunci,Zhao, Zhe,Zhang, Qiya. 2013

[14]A wheat PI4K gene whose product possesses threonine autophophorylation activity confers tolerance to drought and salt in Arabidopsis. Liu, Pei,Xu, Zhao-Shi,Lu, Pan-Pan,Hu, Di,Chen, Ming,Li, Lian-Cheng,Ma, You-Zhi. 2013

[15]Gene Cloning, Overexpression, and Characterization of a Xylanase from Penicillium sp CGMCC 1669. Liu, Wanli,Shi, Pengjun,Yang, Peilong,Wang, Guozeng,Wang, Yaru,Luo, Huiying,Yao, Bin,Liu, Wanli,Chen, Qiang. 2010

[16]Transcript characteristic of myostatin in sheep fibroblasts. Lu, Jian,Ding, Jiatong,Li, Bichun,Lu, Jian,Zhang, Xiaoning,Zhao, Fuping,Zhou, Xinlei,Zhang, Li,Wei, Caihong,Du, Lixin,Ren, Hangxing,Sheng, Xihui,Li, Shangang.

[17]Overexpression of a Lotus corniculatus AP2/ERF transcription factor gene, LcERF080, enhances tolerance to salt stress in transgenic Arabidopsis. Sun, Zhan-Min,Zhou, Mei-Liang,Tang, Yi-Xiong,Wu, Yan-Min,Sun, Zhan-Min,Xiao, Xing-Guo.

[18]The role of thionins in rice defence against root pathogens. Gheysen, Godelieve,Ullah, Chhana,Verbeek, Ruben,Shang, Chenjing,Kyndt, Tina,Ji, Hongli,Verbeek, Ruben,De Vleesschauwer, David,Hofte, Monica.

[19]Molecular cloning, characterization, and function analysis of a mevalonate pyrophosphate decarboxylase gene from Ganoderma lucidum. Shi, Liang,Qin, Lei,Ren, Ang,Fang, Xing,Mu, Dashuai,Zhao, Mingwen,Xu, Yingjie,Tan, Qi.

[20]Increased salt tolerance with overexpression of cation/proton antiporter 1 genes: a meta-analysis. Ma, Yuan-Chun,Ma, Yuan-Chun,Dong, Chao,Cheng, Zong-Ming (Max),Ma, Yuan-Chun,Auge, Robert M.,Cheng, Zong-Ming (Max). 2017

作者其他论文 更多>>

微信小程序