Rice black streaked dwarf virus P7-2 forms a SCF complex through binding to Oryza sativa SKP1-like proteins, and interacts with GID2 involved in the gibberellin pathway

文献类型： 外文期刊

第一作者： Tao, Tao

作者： Tao, Tao;Zhou, Cui-Ji;Chen, Xiang-Ru;Sun, Qian;Zhao, Tian-Yu;Ye, Jian-Chun;Wang, Ying;Zhang, Zong-Ying;Zhang, Yong-Liang;Guo, Ze-Jian;Wang, Xian-Bing;Li, Da-Wei;Yu, Jia-Lin;Han, Cheng-Gui;Tao, Tao;Zhou, Cui-Ji;Chen, Xiang-Ru;Sun, Qian;Zhao, Tian-Yu;Ye, Jian-Chun;Wang, Ying;Zhang, Zong-Ying;Zhang, Yong-Liang;Guo, Ze-Jian;Wang, Xian-Bing;Li, Da-Wei;Yu, Jia-Lin;Han, Cheng-Gui;Wang, Qian

作者机构：

期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）

ISSN： 1932-6203

年卷期： 2017 年 12 卷 5 期

页码：

收录情况： SCI

摘要： As a core subunit of the SCF complex that promotes protein degradation through the 26S proteasome, S-phase kinase-associated protein 1 (SKP1) plays important roles in multiple cellular processes in eukaryotes, including gibberellin (GA), jasmonate, ethylene, auxin and light responses. P7-2 encoded by Rice black streaked dwarf virus (RBSDV), a devastating viral pathogen that causes severe symptoms in infected plants, interacts with SKP1 from different plants. However, whether RBSDV P7-2 forms a SCF complex and targets host proteins is poorly understood. In this study, we conducted yeast two-hybrid assays to further explore the interactions between P7-2 and 25 type I Oryza sativa SKP1-like (OSK) proteins, and found that P7-2 interacted with eight OSK members with different binding affinity. Co-immunoprecipitation assay further confirmed the interaction of P7-2 with OSK1, OSK5 and OSK20. It was also shown that P7-2, together with OSK1 and O. sativa Cullin-1, was able to form the SCF complex. Moreover, yeast two-hybrid assays revealed that P7-2 interacted with gibberellin insensitive dwarf2 (GID2) from rice and maize plants, which is essential for regulating the GA signaling pathway. It was further demonstrated that the N-terminal region of P7-2 was necessary for the interaction with GID2. Overall, these results indicated that P7-2 functioned as a component of the SCF complex in rice, and interaction of P7-2 with GID2 implied possible roles of the GA signaling pathway during RBSDV infection.

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