Simultaneous Determination of Plant Growth Regulators in Fruit by Ultra-performance Liquid Chromatography-Tandem Mass Spectrometry Coupled with Modified QuEChERS Procedure

文献类型： 外文期刊

第一作者： Yan Zhen

作者： Yan Zhen;Nie Ji-Yun;Cheng Yang;Li Zhi-Xia;Xu Guo-Feng;Li Hai-Fei

作者机构：

关键词： Ultra-performance liquid chromatography-tandem mass spectrometry;Plant growth regulators;Fruit

期刊名称：CHINESE JOURNAL OF ANALYTICAL CHEMISTRY （ 影响因子：1.134； 五年影响因子：0.909 ）

ISSN： 0253-3820

年卷期： 2017 年 45 卷 5 期

页码：

收录情况： SCI

摘要： Five plant growth regulators, uniconazole, 6-benzyladenine, kinetin, 4-chlorophenoxyacetic acid and diethyl aminoethyl hexanoate, frequently used in apple, grape, strawberry, peach and orange, were determined by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). A modified QuEChERS (Quick, easy, cheap, effective, rugged and safe) procedure was used in sample pretreatment, which involved extraction with acetonitrile containing 1% acetic acid and clean-up with a mixture of ODS and MgSO4. Mass spectrometric determination was performed in multiple reaction monitoring mode (MRM) using external standards for calibration. The results indicated that the calibration curves for the target analytes were linear in the range of 1-100 mu g L-1 with correlation coefficients greater than 0.999. The recoveries at three spiked levels ranged from 71.82% to 109.24% with RSDs from 0.57% to 12.43%. The respective limits of detection (LOD) and limits of quantification ( LOQ) were 0.15-0.45 mu g kg(-1) and 0.51-1.50 mu g kg(-1) for uniconazole, 0.28-0.53 mu g kg(-1) and 0.94-1.75 mu g kg(-1) for 6-benzyladenine (6-BA), 0.15-69 mu g kg(-1) and 0.51-2.29 mu g kg(-1) for kinetin (6-KT), 0.22-0.60 mu g kg(-1) and 0.74-2.01 mu g kg(-1) for 4-chlorophenoxyacetic acid (4-CPA), 0.88-1.89 mu g kg(-1) and 2.89-6.29 mu g kg(-1) for diethyl aminoethyl hexanoate (DA-6).

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