Rapid generation of genetic diversity by multiplex CRISPR/Cas9 genome editing in rice

文献类型： 外文期刊

第一作者： Shen, Lan

作者： Shen, Lan;Hua, Yufeng;Fu, Yaping;Liu, Qing;Jiao, Xiaozhen;Wang, Junjie;Wang, Kejian;Shen, Lan;Yan, Changjie;Li, Jian;Xin, Gaowei;Wang, Xingchun

作者机构：

关键词： CRISPR/Cas9;genome editing;agronomic genes;rice

期刊名称：SCIENCE CHINA-LIFE SCIENCES （ 影响因子：6.038； 五年影响因子：4.754 ）

ISSN： 1674-7305

年卷期： 2017 年 60 卷 5 期

页码：

收录情况： SCI

摘要： The clustered regularly interspaced short palindromic repeats (CRISPR)-associated endonuclease 9 (CRISPR/Cas9) system has emerged as a promising technology for specific genome editing in many species. Here we constructed one vector targeting eight agronomic genes in rice using the CRISPR/Cas9 multiplex genome editing system. By subsequent genetic transformation and DNA sequencing, we found that the eight target genes have high mutation efficiencies in the T-0 generation. Both heterozygous and homozygous mutations of all editing genes were obtained in T-0 plants. In addition, homozygous sextuple, septuple, and octuple mutants were identified. As the abundant genotypes in T-0 transgenic plants, various phenotypes related to the editing genes were observed. The findings demonstrate the potential of the CRISPR/Cas9 system for rapid introduction of genetic diversity during crop breeding.

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