Evaluation of immunogenicity and protective efficacy of recombinant outer membrane proteins of Haemophilus parasuis serovar 5 in a murine model

文献类型： 外文期刊

第一作者： Li, Miao

作者： Li, Miao;Cai, Ru-Jian;Song, Shuai;Jiang, Zhi-Yong;Li, Yan;Gou, Hong-Chao;Chu, Pin-Pin;Li, Chun-Ling;Li, Miao;Cai, Ru-Jian;Song, Shuai;Jiang, Zhi-Yong;Li, Yan;Gou, Hong-Chao;Chu, Pin-Pin;Li, Chun-Ling;Li, Miao;Cai, Ru-Jian;Song, Shuai;Jiang, Zhi-Yong;Li, Yan;Gou, Hong-Chao;Chu, Pin-Pin;Li, Chun-Ling;Qiu, Hua-Ji

作者机构：

期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）

ISSN： 1932-6203

年卷期： 2017 年 12 卷 4 期

页码：

收录情况： SCI

摘要： Glasser's disease is an economically important infectious disease of pigs caused by Haemophilus parasuis. Few vaccines are currently available that could provide effective cross-protection against various serovars of H. parasuis. In this study, five OMPs (OppA, TolC, HxuC, LppC, and HAPS_0926) identified by bioinformatic approaches, were cloned and expressed as recombinant proteins. Antigenicity of the purified proteins was verified through Western blotting, and primary screening for protective potential was evaluated in vivo. Recombinant TolC (rTolC), rLppC, and rHAPS_0926 proteins showing marked protection of mice against H. parasuis infection, and were further evaluated individually or in combination. Mice treated with these three OMPs produced humoral and host cell-mediated responses, with a significant rise in antigen-specific IgG titer and lymphoproliferative response in contrast with the mock-immunized group. Significant increases were noted in CD4(+), CD8(+) T cells, and three cytokines (IL-2, IL-4, and IFN-(Y)) in vaccinated animals. The antisera against candidate antigens could efficiently impede bacterial survival in whole blood bactericidal assay against H. parasuis infection. The multi-protein vaccine induced more pronounced immune responses and offered better protection than individual vaccines. Our findings indicate that these three OMPs are promising antigens for the development of multi-component subunit vaccines against Glasser's disease.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序