A novel colloidal gold immunochromatography assay strip for the diagnosis of schistosomiasis japonica in domestic animals

文献类型： 外文期刊

第一作者： Xu, Rui

作者： Xu, Rui;Feng, Jintao;Hong, Yang;Lv, Chao;Zhao, Dengyun;Lin, Jiaojiao;Lu, Ke;Li, Hao;Liu, Jinming;Cao, Xiaodan;Wang, Tao;Zai, Jinli;Wang, Zhaozhe;Jia, Bingguang;Han, Qian;Zhu, Chuangang;Lin, Jiaojiao

作者机构：

关键词： Colloidal gold immunochromatography assay strip;Schistosoma japonicum;Recombinant streptococcal protein G;Immunodiagnosis

期刊名称：INFECTIOUS DISEASES OF POVERTY （ 影响因子：4.52； 五年影响因子：4.307 ）

ISSN： 2049-9957

年卷期： 2017 年 6 卷

页码：

收录情况： SCI

摘要： Background: Schistosomiasis remains a major public health concern in China and an epidemiological survey has revealed that schistosome-infected bovines and goats are the main transmission sources for the disease. Therefore, development of a sensitive technique for the diagnosis of schistosomiasis in domestic animals is necessary. Method: A novel colloidal gold immunochromatography assay (GICA) strip was developed for detecting Schistosoma japonicum in domestic animals. The colloidal gold was conjugated with recombinant streptococcal protein G (rSPG). As the test and control lines, the schistosome soluble egg antigen and rSPG, respectively, were blotted on nitrocellulose membrane. Results: The lowest detectable serum dilution was 1: 640 for schistosome-infected buffaloes. The cross-reaction rate of GICA was 14.29% with Paramphistomum sp. in buffaloes, 16.67% with Haemonchus sp. in goats, and 33.33% with Orientobilharzia sp. in goats. These results were slightly lower and similar to those obtained through ELISA. Moreover, the strips for detecting S. japonicum in mice, rabbits, buffaloes, and goats showed high sensitivity (100.00%, 100.00%, 100.00%, and 100.00%, respectively) and specificity (100.00%, 100.00%, 94.23%, and 88.64%, respectively). And the sensitivity or specificity of the GICA strips did not present any significant differences after storage for 12 months at room temperature. When compared with ELISA, the GICA strips exhibited similar sensitivity and specificity in the diagnosis of schistosomiasis in mice, rabbits, buffaloes, and goats. Besides, only 5 mu l of serum are required for the test and the detection can be completed within 5 min. Conclusion: This study is the first to develop a GICA strip using gold-rSPG conjugate for the diagnosing of schistosomiasis in domestic animals, and preliminary results showed that the developed strip may be suitable for large-scale screening of schistosomiasis in endemic areas.

分类号：

  • 相关文献

[1]Molecular characterization of SjBIRP, another apoptosis inhibitor, from Schistosoma japonicum. Dao, Jinwei,Zhu, Lihui,Luo, Rong,Hu, Chao,Wang, Yuqing,Li, Hao,Lu, Ke,Liu, Jinming,Lin, Jiaojiao,Cheng, Guofeng,Dao, Jinwei,Cheng, Guofeng. 2014

[2]Cloning and expression of gynecophoral canal protein gene of Schistosoma japonicum (Chinese strain). Jin, YM,Lin, JJ,Feng, XG,Zhang, L,Wu, XF,Zhou, YC,Cai, YM. 2002

[3]Cloning and expression of actin gene of Schistosoma japonicum Chinese strain. Zhu, JG,Lin, JJ,Feng, XG,Wu, XF,Zhou, YC,Cai, YM. 2000

[4]Proteomic analysis of differentially expressed proteins between the male and female worm of Schistosomal japonicum after pairing. Cheng, GF,Lin, JJ,Feng, XG,Fu, ZQ,Jin, YM,Yuan, CX,Zhou, YC,Cai, YM. 2005

[5]Production and testing of Schistosoma japonicum candidate vaccine antigens in the natural ovine host. Taylor, MG,Huggins, MC,Shi, FH,Lin, JJ,Tian, E,Ye, P,Shen, W,Qian, CG,Lin, BF,Bickle, QD. 1998

[6]Studies on the expression of the 28kD glutathione S-transferase gene from Schistosoma japonicum in the silkworm (Bombyx mori) larvae and insect cells. Tian, E,Yang, GZ,Cai, YM,Shi, FH,Wu, XF. 1997

[7]Analysis of early hepatic stage schistosomula gene expression by subtractive expressed sequence tags library. Wang, Xinzhi,Feng, XinGang,Fu, Zhiqiang,Jin, Yamei,Peng, Jinbiao,Lin, JiaoJiao,Gobert, Geoffrey N.. 2009

[8]Gene cloning, expression and vaccine testing of Schistosoma japonicum SjFABP. Liu, JM,Cai, XZ,Lin, JJ,Fu, ZQ,Yang, GZ,Shi, FH,Cai, YM,Shen, W,Taylor, MG,Wu, XF. 2004

[9]A specific PCR assay for the identification and differentiation of Schistosoma japonicum geographical isolates in mainland China based on analysis of mitochondrial genome sequences. Zhao, Guang-Hui,Li, Juan,Song, Hui-Qun,Li, Xiao-Yan,Zou, Feng-Cai,Zhu, Xing-Quan,Zhao, Guang-Hui,Zhao, Guang-Hui,Li, Juan,Song, Hui-Qun,Chen, Fen,Lin, Rui-Qing,Yuan, Zi-Guo,Weng, Ya-Biao,Li, Xiao-Yan,Zou, Feng-Cai,Zhu, Xing-Quan,Hu, Min,Zhu, Xing-Quan.

[10]Molecular characterization and expression profile of nanos in Schistosoma japonicum and its influence on the expression several mammalian stem cell factors. Giri, Bikash Ranjan,Du, Xiaoli,Xia, Tianqi,Chen, Yongjun,Li, Hao,Cheng, Guofeng.

[11]Dose-dependent Inhibition of Gynecophoral Canal Protein Gene Expression in Vitro in the Schistosome (Schistosoma japonicum) by RNA Interference. Cheng, GF,Lin, JJ,Shi, Y,Jin, YX,Fu, ZQ,Jin, YM,Zhou, YC,Cai, YM.

[12]Vaccination with recombinant paramyosin in Montanide ISA206 protects against Schistosoma japonicum infection in water buffalo. Wu, Hannah Wei,Pond-tor, Sunthorn,Park, Sangshin,Kurtis, Jonathan D.,Fu, Zhi-Qiang,Lu, Ke,Hong, Yang,Li, Hao,Liu, Jin-Ming,Lin, Jiao-Jiao,Meng, Rui,Chu, Kai,Hou, Ming,Jiz, Mario,Meng, Rui,Chu, Kai.

[13]Comparative analysis of microRNA in schistosomula isolated from non-permissive host and susceptible host. Han, Hongxiao,Peng, Jinbiao,Hong, Yang,Fu, Zhiqiang,Lu, Ke,Li, Hao,Zhu, Chuangang,Lin, Jiaojiao,Lin, Jiaojiao,Han, Hongxiao,Zhao, Qiuhua.

[14]iTRAQ-based comparative proteomic analysis of excretory-secretory proteins of schistosomula and adult worms of Schistosoma japonicum. Cao, Xiaodan,Fu, Zhiqiang,Han, Hongxiao,Han, Qian,Lu, Ke,Hong, Yang,Lin, Jiaojiao,Zhang, Min,Han, Yanhui,Lin, Jiaojiao.

[15]The molecular characterization and RNAi silencing of SjZFP1 in Schistosoma japonicum. Liu, Xin,Liu, Jin-Ming,Song, Zhen-yu,Xing, Rong-He,Jin, Ya-Mei,Guo, Yan-hong,Li, Hao,Lu, Ke,Shi, Yao-jun,Cheng, Guo-feng,Lin, Jiao-Jiao,Lin, Jiao-Jiao.

[16]Identification of Th1 epitopes within molecules from the lung-stage schistosomulum of Schistosoma japonicum by combining prediction analysis of the transcriptome with experimental validation. Zhang, Yan Li,Jia, Kan,Zhao, Ben Peng,Li, Ying,Yuan, Chun Xiu,Yang, Jian Mei,Lin, Jiao Jiao,Feng, Xin Gang,Jia, Kan,Zhao, Ben Peng,Li, Ying.

[17]A candidate recombinant antigen for diagnosis of schistosomiasis japonica in domestic animals. Feng, Jintao,Xu, Rui,Zhang, Xin,Han, Yu,He, Chuanchuan,Lu, Chao,Hong, Yang,Lu, Ke,Li, Hao,Jin, Yamei,Lin, Jiaojiao,Liu, Jinming,Lin, Jiaojiao.

[18]Identification and functional characterization of Oncomelania hupensis macrophage migration inhibitory factor involved in the snail host innate immune response to the parasite Schistosoma japonicum. Huang, Shuaiqin,Cao, Yunchao,Lu, Mingke,Peng, Wenfeng,Tang, Chongti,Tang, Liang,Huang, Shuaiqin,Cao, Yunchao,Lu, Mingke,Peng, Wenfeng,Tang, Chongti,Tang, Liang,Lin, Jiaojiao.

[19]A retinoid X receptor (RXR1) homolog from Schistosoma japonicum: Its ligand-binding domain may bind to 9-cis-retinoic acid. Qiu, Chunhui,Fu, Zhiqiang,Shi, Yaojun,Hong, Yang,Lin, Jiaojiao,Qiu, Chunhui,Liu, Shengfa.

[20]Apoptosis phenomenon in the schistosomulum and adult worm life cycle stages of Schistosoma japonicum. Han, Hongxiao,Xu, Jinjun,Tao, Jianping,Han, Hongxiao,Hong, Yang,Zhang, Min,Han, Yanhui,Fu, Zhiqiang,Shi, Yaojun,Lin, Jiaojiao,Gobert, Geoffrey N.,Peng, Jinbiao.

作者其他论文 更多>>

微信小程序