Characterization of Brucella abortus mutant strain Delta 22915, a potential vaccine candidate

文献类型： 外文期刊

第一作者： Bao, Yanqing

作者： Bao, Yanqing;Tian, Mingxing;Li, Peng;Liu, Jiameng;Ding, Chan;Yu, Shengqing;Yu, Shengqing

作者机构：

期刊名称：VETERINARY RESEARCH （ 影响因子：3.683； 五年影响因子：4.106 ）

ISSN： 0928-4249

年卷期： 2017 年 48 卷

页码：

收录情况： SCI

摘要： Brucellosis, caused by Brucella spp., is an important zoonosis worldwide. Vaccination is an effective strategy for protection against Brucella infection in livestock in developing countries and in wildlife in developed countries. However, current vaccine strains including S19 and RB51 are pathogenic to humans and pregnant animals, limiting their use. In this study, we constructed the Brucella abortus (B. abortus) S2308 mutant strain Delta 22915, in which the putative lytic transglycosylase gene BAB_RS22915 was deleted. The biological properties of mutant strain Delta 22915 were characterized and protection of mice against virulent S2308 challenge was evaluated. The mutant strain Delta 22915 showed reduced survival within RAW264.7 cells and survival in vivo in mice. In addition, the mutant strain Delta 22915 failed to escape fusion with lysosomes within host cells, and caused no observable pathological damage. RNA-seq analysis indicated that four genes associated with amino acid/nucleotide transport and metabolism were significantly upregulated in mutant strain Delta 22915. Furthermore, inoculation of Delta 22915 at -105 colony forming units induced effective host immune responses and long-term protection of BALB/c mice. Therefore, mutant strain Delta 22915 could be used as a novel vaccine candidate in the future to protect animals against B. abortus infection.

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