Identification of Drought-Responsive MicroRNAs from Roots and Leaves of Alfalfa by High-Throughput Sequencing

文献类型： 外文期刊

第一作者： Li, Yue

作者： Li, Yue;Wan, Liqiang;Bi, Shuyi;Wan, Xiufu;Li, Zhenyi;Cao, Jing;Tong, Zongyong;Xu, Hongyu;He, Feng;Li, Xianglin

作者机构：

关键词： alfalfa;drought;microRNA;small RNA;differential expression

期刊名称：GENES （ 影响因子：4.096； 五年影响因子：4.339 ）

ISSN： 2073-4425

年卷期： 2017 年 8 卷 4 期

页码：

收录情况： SCI

摘要： Alfalfa, an important forage legume, is an ideal crop for sustainable agriculture and a potential crop for bioenergy resources. Drought, one of the most common environmental stresses, substantially affects plant growth, development, and productivity. MicroRNAs ( miRNAs) are newly discovered gene expression regulators that have been linked to several plant stress responses. To elucidate the role of miRNAs in drought stress regulation of alfalfa, a high-throughput sequencing approach was used to analyze 12 small RNA libraries comprising of four samples, each with three biological replicates. From the 12 libraries, we identified 348 known miRNAs belonging to 80 miRNA families, and 281 novel miRNAs, using Mireap software. Eighteen known miRNAs in roots and 12 known miRNAs in leaves were screened as drought-responsive miRNAs. With the exception of miR319d and miR157a which were upregulated under drought stress, the expression pattern of drought-responsive miRNAs was different between roots and leaves in alfalfa. This is the first study that has identified miR3512, miR3630, miR5213, miR5294, miR5368 and miR6173 as drought-responsive miRNAs. Target transcripts of drought-responsive miRNAs were computationally predicted. All 447 target genes for the known miRNAs were predicted using an online tool. This study provides a significant insight on understanding drought-responsive mechanisms of alfalfa.

分类号：

  • 相关文献

[1]Novel microRNAs uncovered by deep sequencing of small RNA transcriptomes in bread wheat (Triticum aestivum L.) and Brachypodium distachyon (L.) Beauv. Wei, Bo,Zhang, Rongzhi,Li, Aili,Jia, Jizeng,Mao, Long,Wei, Bo,Zhang, Rongzhi,Li, Aili,Jia, Jizeng,Mao, Long,Cai, Tao,Li, Shan,Qi, Yijun,Huo, Naxin,Gu, Yong Q.,Vogel, John.

[2]Genome-wide sequencing of small RNAs reveals a tissue-specific loss of conserved microRNA families in Echinococcus granulosus. Bai, Yun,Jin, Lei,Kang, Hui,Zhu, Yongqiang,Zhang, Lu,Li, Xia,Ma, Fengshou,Wang, Shengyue,Zhang, Zhuangzhi,Zhao, Li,Shi, Baoxin,Li, Jun,Zhang, Wenbao,McManus, Donald P.. 2014

[3]Characterization and profiling of MicroRNAs in posterior silk gland of the silkworm (Bombyx mori). Song, Fei,Wang, Xin,Chen, Chen,Fan, Yangyang,Tang, Shunming,Huang, Jinshan,Guo, Xijie,Shen, Xingjia,Tang, Shunming,Huang, Jinshan,Guo, Xijie,Shen, Xingjia.

[4]High-Throughput Sequencing of Small RNA Transcriptomes in Maize Kernel Identifies miRNAs Involved in Embryo and Endosperm Development. Xing, Lijuan,Zhu, Ming,Zhang, Min,Li, Wenzong,Zou, Junjie,Wang, Lei,Xu, Miaoyun,Zhu, Ming,Jiang, Haiyang. 2017

[5]Arabidopsis enhanced drought tolerance1/HOMEODOMAIN GLABROUS11 confers drought tolerance in transgenic rice without yield penalty. Yu, Linhui,Chen, Xi,Wang, Zhen,Xiang, Chengbin,Wang, Shimei,Zhu, Qisheng,Wang, Yuping,Li, Shigui,Wang, Shimei,Zhu, Qisheng.

[6]Identification of Hop stunt viroid infecting Citrus limon in China using small RNAs deep sequencing approach. Fu, Shuai,Qian, Yajuan,Xu, Yi,Zhou, Xueping,Zhou, Xueping. 2015

[7]Mutation of the RDR1 gene caused genome-wide changes in gene expression, regional variation in small RNA clusters and localized alteration in DNA methylation in rice. Wang, Ningning,Zhang, Di,Wang, Zhenhui,Xun, Hongwei,Wang, Hui,Huang, Wei,Liu, Ying,Li, Ning,Ou, Xiufang,Zhang, Chunyu,Liu, Bao,Wang, Ningning,Ma, Jian,Lin, Xiuyun,Zhang, Chunyu,Wang, Ningning,Wang, Ming-Bo. 2014

[8]Phytophthora effector targets a novel component of small RNA pathway in plants to promote infection. Qiao, Yongli,Shi, Jinxia,Zhai, Yi,Hou, Yingnan,Ma, Wenbo,Qiao, Yongli,Shi, Jinxia,Hou, Yingnan,Ma, Wenbo,Qiao, Yongli.

[9]High-Level Accumulation of Exogenous Small RNAs Not Affecting Endogenous Small RNA Biogenesis and Function in Plants. Shen Wan-xia,Zhou Chang-yong,Shen Wan-xia,Smith, Neil A.,Wang Ming-bo,Shen Wan-xia,Zhou Chang-yong. 2014

[10]Micropathogen Community Analysis in Hyalomma rufipes via High-Throughput Sequencing of Small RNAs. Luo, Jin,Liu, Min-Xuan,Ren, Qiao-Yun,Chen, Ze,Tian, Zhan-Cheng,Hao, Jia-Wei,Wu, Feng,Liu, Xiao-Cui,Luo, Jian-Xun,Yin, Hong,Wang, Hui,Liu, Guang-Yuan,Liu, Min-Xuan,Yin, Hong,Wang, Hui,Wang, Hui. 2017

[11]Mercury toxicity, molecular response and tolerance in higher plants. Yang, Zhi Min,Chen, Jian. 2012

[12]Genome-Wide Characterization of Rice Black Streaked Dwarf Virus-Responsive MicroRNAs in Rice Leaves and Roots by Small RNA and Degradome Sequencing. Sun, Zongtao,He, Yuqing,Li, Junmin,Wang, Xu,Chen, Jianping,He, Yuqing.

[13]Heritable variation and small RNAs in the progeny of chimeras of Brassica juncea and Brassica oleracea. Li, Junxing,Wang, Yan,Zhang, Langlang,Liu, Bin,Cao, Liwen,Qi, Zhenyu,Chen, Liping,Li, Junxing,Zhang, Langlang,Liu, Bin,Cao, Liwen,Chen, Liping,Wang, Yan,Qi, Zhenyu.

[14]A CC-NBS-LRR type gene GHNTR1 confers resistance to southern root-knot nematode in Nicotiana.benthamiana and Nicotiana.tabacum. Zhang, Baolong,Zhang, Wenhua,Zhang, Baolong,Yang, Yuwen,Wang, Jinyan,Ling, Xitie,Hu, Zhongze,Liu, Tingli,Chen, Tianzi.

[15]Characterization of microRNAs from sheep (Ovis aries) using computational and experimental analyses. Sheng, Xihui,Wei, Caihong,Liu, Tao,Zhang, Li,Du, Lixin,Sheng, Xihui,Song, Xuemei,Yu, Yan,Niu, Lili,Li, Shangang,Li, Hongbin.

[16]Characterization of the promoter of phosphate transporter TaPHT1.2 differentially expressed in wheat varieties. Miao, Jun,Sun, Jinghan,Liu, Dongcheng,Li, Bin,Zhang, Aimin,Li, Zhensheng,Tong, Yiping,Miao, Jun. 2009

[17]The expression of pectin methylesterase in onion flower buds is associated with the dominant male-fertility restoration allele. Huo, Yumeng,Miao, Jun,Liu, Bingjiang,Yang, Yanyan,Zhang, Yihui,He, Qiwei,Wu, Xiong,Huo, Yumeng,Miao, Jun,Liu, Bingjiang,Yang, Yanyan,Zhang, Yihui,He, Qiwei,Wu, Xiong,Huo, Yumeng,Miao, Jun,Liu, Bingjiang,Yang, Yanyan,Zhang, Yihui,He, Qiwei,Wu, Xiong,Tahara, Yasuki,Meng, Qingwei,Kitano, Hidemi. 2012

[18]The Differential Expression of Resistant Genes Related to Cucumber Scab Displayed by cDNA-AFLP. Sun Xun,Li Xixiang,Qiu Yang,Shen Di,Song Jiangping,Sun Xun,Ren Ruixing. 2010

[19]A key structural gene, AaLDOX, is involved in anthocyanin biosynthesis in all red-fleshed kiwifruit (Actinidia arguta) based on transcriptome analysis. Li, Yukuo,Fang, Jinbao,Qi, Xiujuan,Lin, Miaomiao,Zhong, Yunpeng,Sun, Leiming. 2018

[20]Identification and Differential Expression of Biomineralization Genes in the Mantle of Pearl Oyster Pinctada fucata. Li, Haimei,Yu, Dahui,Li, Haimei,Yu, Dahui,Li, Haimei,Zhang, Bo,Fan, Sigang,Liu, Baosuo,Su, Jiaqi,Yu, Dahui,Yu, Dahui,Yu, Dahui.

作者其他论文 更多>>

微信小程序