The Interactions between ZnO Nanoparticles (NPs) and alpha-Linolenic Acid (LNA) Complexed to BSA Did Not Influence the Toxicity of ZnO NPs on HepG2 Cells
文献类型: 外文期刊
第一作者: Zhou, Yiwei
作者: Zhou, Yiwei;Fang, Xin;Xiao, Aiping;Liu, Liangliang;Cao, Yi;Zhou, Yiwei;Fang, Xin;Gong, Yu;Xie, Yixi;Cao, Yi
作者机构:
关键词: HepG2 cells;alpha-linolenic acid (LNA);ZnO nanoparticles;interaction
期刊名称:NANOMATERIALS ( 影响因子:5.076; 五年影响因子:5.346 )
ISSN: 2079-4991
年卷期: 2017 年 7 卷 4 期
页码:
收录情况: SCI
摘要: Background: Nanoparticles (NPs) entering the biological environment could interact with biomolecules, but little is known about the interaction between unsaturated fatty acids (UFA) and NPs. Methods: This study used alpha-linolenic acid (LNA) complexed to bovine serum albumin (BSA) for UFA and HepG2 cells for hepatocytes. The interactions between BSA or LNA and ZnO NPs were studied. Results: The presence of BSA or LNA affected the hydrodynamic size, zeta potential, UV-Vis, fluorescence, and synchronous fluorescence spectra of ZnO NPs, which indicated an interaction between BSA or LNA and NPs. Exposure to ZnO NPs with the presence of BSA significantly induced the damage to mitochondria and lysosomes in HepG2 cells, associated with an increase of intracellular Zn ions, but not intracellular superoxide. Paradoxically, the release of inflammatory cytokine interleukin-6 (IL-6) was decreased, which indicated the anti-inflammatory effects of ZnO NPs when BSA was present. The presence of LNA did not significantly affect all of these endpoints in HepG2 cells exposed to ZnO NPs and BSA. Conclusions: the results from the present study indicated that BSA-complexed LNA might modestly interact with ZnO NPs, but did not significantly affect ZnO NPs and BSA-induced biological effects in HepG2 cells.
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