Protocols for Investigating the Host-tissue Distribution, Transmission-mode, and Effect on the Host Fitness of a Densovirus in the Cotton Bollworm
文献类型: 外文期刊
第一作者: Yuan, He
作者: Yuan, He;Wu, Kongming;Xu, Pengjun;Graham, Robert I.
作者机构:
关键词: Immunology;Issue 122;densovirus;cotton bollworm;interaction;tissue distribution;horizontal transmission;vertical transmission;bioassays
期刊名称:JOVE-JOURNAL OF VISUALIZED EXPERIMENTS ( 影响因子:1.355; 五年影响因子:1.696 )
ISSN: 1940-087X
年卷期: 2017 年 122 期
页码:
收录情况: SCI
摘要: Many novel viruses have been discovered in animal hosts using next-generation sequencing technologies. Previously, we reported a mutualistic virus, Helicoverpa armigera densovirus (HaDV2), in a lepidopteran species, the cotton bollworm, Helicoverpa armigera (Hubner). Here, we describe the protocols that are currently used to study the effect of HaDV2 on its host. First, we establish a HaDV2-free cotton bollworm colony from a single breeding pair. Then, we orally inoculate some neonate larval offspring with HaDV2-containing filtered liquid to produce two colonies with the same genetic background: one HaDV2- infected, the other uninfected. A protocol to compare life table parameters (e.g., larval, pupal, and adult periods and fecundity) between the HaDV2- infected and -uninfected individuals is also presented, as are the protocols for determining the host-tissue distribution and transmission efficiency of HaDV2. These protocols would also be suitable for investigating the effects of other orally transmitted viruses on their insect hosts, lepidopteran hosts in particular.
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