Molecular cloning, characterization and antigenicity of Babesia sp BQ1 (Lintan) (Babesia cf. motasi) apical membrane antigen-1 (AMA-1)

文献类型: 外文期刊

第一作者: Niu, Qingli

作者: Niu, Qingli;Liu, Zhijie;Yang, Jifei;Guan, Guiquan;Pan, Yuping;Luo, Jianxun;Yin, Hong;Yin, Hong

作者机构:

关键词: Babesia sp;BQ1 (Lintan);AMA-1;characterization;expression

期刊名称:PARASITOLOGY ( 影响因子:3.234; 五年影响因子:3.118 )

ISSN: 0031-1820

年卷期: 2017 年 144 卷 5 期

页码:

收录情况: SCI

摘要: Apical membrane antigen-1 (AMA-1) has been described as a potential vaccine candidate in apicomplexan parasites. Here we characterize the ama-1 gene. The full-length ama-1 gene of Babesia sp. BQ1 (Lintan) (BLTAMA-1) is 1785 bp, which contains an open reading frame (ORF) encoding a 65-kDa protein of 594 amino acid residues; by definition, the 5 UTR precedes the first methionine of the ORF. Phylogenetic analysis based on AMA-1 amino acid sequences clearly separated Piroplasmida from other Apicomplexa parasites. The Babesia sp. BQ1 (Lintan) AMA-1 sequence is most closely associated with that of B. ovata and B. bigemina, with high bootstrap value. A recombinant protein encoding a conserved region and containing ectodomains I and II of BLTAMA-1 was constructed. BLTrAMA-1-DI/DII proteins were tested for reactivity with sera from sheep infected by Babesia sp. BQ1 (Lintan). In Western-blot analysis, native Babesia sp. BQ1 (Lintan) AMA-1 proteins were recognized by antibodies raised in rabbits against BLTrAMA-1 in vitro. The results of this study are discussed in terms of gene characterization, taxonomy and antigenicity.

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