De novo DNA methylation during monkey pre-implantation embryogenesis

文献类型： 外文期刊

第一作者： Niu, Yuyu

作者： Niu, Yuyu;Sun, Yi Eve;Chen, Yongchang;Kang, Yu;Si, Chenyang;Li, Chang;Sun, Nianqin;Si, Wei;Wang, Hong;Ji, Weizhi;Tan, Tao;Gao, Fei;Lu, Hanlin;Niu, Yuyu;Chen, Yongchang;Kang, Yu;Si, Chenyang;Li, Chang;Sun, Nianqin;Si, Wei;Wang, Hong;Ji, Weizhi;Tan, Tao;Sun, Yi Eve;Li, Siguang;Luo, Yuping;Sun, Yi Eve;Yu, Juehua

作者机构：

关键词： rhesus monkey;pre-implantation embryogenesis;de novo DNA methylation

期刊名称：CELL RESEARCH （ 影响因子：25.617； 五年影响因子：25.924 ）

ISSN： 1001-0602

年卷期： 2017 年 27 卷 4 期

页码：

收录情况： SCI

摘要： Critical epigenetic regulation of primate embryogenesis entails DNA methylome changes. Here we report genome-wide composition, patterning, and stage-specific dynamics of DNA methylation in pre-implantation rhesus monkey embryos as well as male and female gametes studied using an optimized tagmentation-based whole-genome bisulfite sequencing method. We show that upon fertilization, both paternal and maternal genomes undergo active DNA demethylation, and genome-wide de novo DNA methylation is also initiated in the same period. By the 8-cell stage, remethylation becomes more pronounced than demethylation, resulting in an increase in global DNA methylation. Promoters of genes associated with oxidative phosphorylation are preferentially remethylated at the 8-cell stage, suggesting that this mode of energy metabolism may not be favored. Unlike in rodents, X chromosome inactivation is not observed during monkey pre-implantation development. Our study provides the first comprehensive illustration of the 'wax and wane' phases of DNA methylation dynamics. Most importantly, our DNA methyltransferase loss-of-function analysis indicates that DNA methylation influences early monkey embryogenesis.

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