Deletion of the B125R gene in the African swine fever virus SY18 strain leads to an A104R frameshift mutation slightly attenuating virulence in domestic pigs

文献类型： 外文期刊

第一作者： Zhu, Rongnian

作者： Zhu, Rongnian;Wang, Ying;Zhang, Han;Yang, Jinjin;Fan, Jiaqi;Zhang, Yanyan;Wang, Yu;Li, Qixuan;Zhou, Xintao;Yue, Huixian;Qi, Yu;Wang, Shuchao;Chen, Teng;Zhang, Shoufeng;Hu, Rongliang

作者机构：

关键词： African swine fever virus; B125R deletion; A104R mutation; Virulence

期刊名称：VIRUS RESEARCH （ 影响因子：5.0； 五年影响因子：4.0 ）

ISSN： 0168-1702

年卷期： 2024 年 343 卷

页码：

收录情况： SCI

摘要： African swine fever (ASF), caused by the ASF virus (ASFV), is a hemorrhagic and fatal viral disease that affects Eurasian wild boars and domestic pigs, posing a substantial threat to the global pig breeding industry. ASFV, a double -stranded DNA virus, possesses a large genome containing up to 160 open reading frames, most of which exhibit unknown functions. The B125R gene of ASFV, located at the 105595 - 105972 bp site in the ASFV-SY18 genome, remains unexplored. In this study, we discovered that B125R deletion did not affect recombinant virus rescue, nor did it hinder viral replication during the intermediate growth phase. Although the virulence of the recombinant strain harboring this deletion was attenuated, intramuscular inoculation of the recombinant virus in pigs at doses of 10 2 or 10 4 TCID 50 resulted in mortality. Moreover, sequencing analysis of six recombinant strains obtained from three independent experiments consistently revealed an adenine insertion at the 47367 - 47375 bp site in the A104R gene due to the B125R deletion, leading to premature termination of this gene. Intriguingly, this insertion did not influence the transcription of the A104R gene between the recombinant and parental strains. Consequently, we postulate that the deletion of the B125R gene in ASFV-SY18 or other genotype II strains may marginally attenuate virulence in domestic pigs.

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