bta-miR-23a involves in adipogenesis of progenitor cells derived from fetal bovine skeletal muscle

文献类型： 外文期刊

第一作者： Guan, Long

作者： Guan, Long;Hu, Xin;Xing, Yishen;Zhou, Zhengkui;Gao, Huijiang;Li, Junya;Zhang, Lupei;Liu, Li;Liang, Xingwei;Yang, Qiyuan;Du, Min;Jin, Shengyun;Bao, Jinshan

作者机构：

期刊名称：SCIENTIFIC REPORTS （ 影响因子：4.379； 五年影响因子：5.133 ）

ISSN： 2045-2322

年卷期： 2017 年 7 卷

页码：

收录情况： SCI

摘要： Intramuscular fat deposition or marbling is essential for high quality beef. The molecular mechanism of adipogenesis in skeletal muscle remains largely unknown. In this study, we isolated Platelet-derived growth factor receptor alpha (PDGFR alpha) positive progenitor cells from fetal bovine skeletal muscle and induced into adipocytes. Using miRNAome sequencing, we revealed that bta-miR-23a was an adipogenic miRNA mediating bovine adipogenesis in skeletal muscle. The expression of bta-miR-23a was down-regulated during differentiation of PDGFR alpha+ progenitor cells. Forced expression of bta-miR-23a mimics reduced lipid accumulation and inhibited the key adipogenic transcription factor peroxisome proliferative activated receptor gamma (PPAR gamma) and CCAAT/enhancer binding protein alpha (C/EBP alpha). Whereas down-regulation of bta-miR-23a by its inhibitors increased lipid accumulation and expression of C/EBP alpha, PPAR gamma and fatty acid-binding protein 4 (FABP4). Target prediction analysis revealed that ZNF423 was a potential target of bta-miR-23a. Dual-luciferase reporter assay revealed that bta-miR-23a directly targeted the 3'-UTR of ZNF423. Together, our data showed that bta-miR-23a orchestrates early intramuscular adipogeneic commitment as an anti-adipogenic regulator which acts by targeting ZNF423.

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