Comparative analysis of pre- and post-parasitic transcriptomes and mining pioneer effectors of Heterodera avenae

文献类型： 外文期刊

第一作者： Yang, Dan

作者： Yang, Dan;Chen, Changlong;Liu, Qian;Jian, Heng;Chen, Changlong

作者机构：

关键词： Heterodera avenae;Pre-parasitic and post-parasitic stage;Transcriptome sequencing;Effectors;Comparative analysis

期刊名称：CELL AND BIOSCIENCE （ 影响因子：7.133； 五年影响因子：6.302 ）

ISSN： 2045-3701

年卷期： 2017 年 7 卷

页码：

收录情况： SCI

摘要： Background: The cereal cyst nematode (CCN, Heterodera avenae) is a devastating pathogen of wheat and barley crops in many countries. We aimed to prioritize genetic and molecular targets for H. avenae control via the powerful and integrative bioinformatics platform. Results: Here, we sequenced mRNA isolated from Chinese H. avenae at pre-parasitic (consisting of egg, J1 and hatched-J2) stages and post-parasitic (consisting of parasitic-J2, J3, J4 and adults) stages. Total 1,066,719 reads of whole life cycle transcriptomes were assembled into 10,811 contigs with N50 length of 1754 bp and 71,401 singletons. Comparative analyses of orthologous among H. avenae and 7 other nematodes with various life-styles revealed the significance and peculiarity of neurological system for sedentary phytonematode. KEGG pathway enrichment demonstrated active crosstalk events of nervous system at pre-parasitic stages, and 6 FMRFamide-like neuropeptides were verified to display an expression peak at the hatched-J2 stage in H. avenae. Furthermore, multiple approaches were undertaken to mine putative effectors and parasitism-specific genes. Notably, H. avenae might represent the first phytonematode reported to possess the pioneer effectors with RxLR motif and potential effectors with homologies to Ant-5/Ant-34. Conclusion: Our work provides valuable resources for in-depth understanding the parasitism and pathogenicity of H. avenae, as well as developing new targets-oriented strategies on effective managements.

分类号：

  • 相关文献

[1]Comparative analysis of two-component signal transduction systems in six Synechococcus strains. Zhang, Xiaowen,Ye, Naihao,Guan, Xiangyu,Qin, Song,Liang, Chengwei. 2009

[2]FTIR Analysis of Burkholderia seminalis from Apricot, Water and Rice Rhizosphere. Fang, Y.,Fang, Y.,Li, B.,Wang, Y. L.,Sun, G. C.,Guo, L. B..

[3]New phylogenomic and comparative analyses provide corroborating evidence that Myxozoa is Cnidaria. Feng, Jin-Mei,Xiong, Jie,Zhang, Jin-Yong,Miao, Wei,Yang, Ya-Lin,Yao, Bin,Zhou, Zhi-Gang,Feng, Jin-Mei.

[4]Comparative analysis of microRNA profiles between adult Ascaris lumbricoides and Ascaris suum. Shao, Chang-Chun,Tao, Jian-Ping,Shao, Chang-Chun,Xu, Min-Jun,Song, Hui-Qun,Zhu, Xing-Quan,Alasaad, Samer,Peng, Lifei. 2014

[5]A comparative analysis of agricultural research and extension reforms in China and India. Babu, Suresh Chandra,Huang, Jikun,Venkatesh, P.,Zhang, Yumei. 2015

[6]Genome-Wide Analysis of the First Sequenced Mycoplasma capricolum subsp capripneumoniae Strain M1601. Chen, Shengli,Hao, Huafang,Zhao, Ping,He, Ying,Gao, Pengcheng,Guo, Han,Ji, Wenheng,Wang, Zhanhui,Lu, Zhongxin,Chu, Yuefeng,Liu, Yongsheng,Thiaucourt, Francois,Thiaucourt, Francois. 2017

[7]The complete mitochondrial genome of Engyodontium album and comparative analyses with Ascomycota mitogenomes. Yuan, Xiao-Long,Mao, Xin-Xin,Liu, Xin-Min,Zhang, Peng,Zhang, Zhong-Feng,Cheng, Sen. 2017

[8]A collection of 10,096 indica rice full-length cDNAs reveals highly expressed sequence divergence between Oryza sativa indica and japonica subspecies. Liu, Xiaohui,Lu, Tingting,Yu, Shuliang,Li, Ying,Huang, Yuchen,Huang, Tao,Zhang, Lei,Zhu, Jingjie,Zhao, Qiang,Fan, Danlin,Mu, Jie,Shangguan, Yingying,Feng, Qi,Guan, Jianping,Ying, Kai,Zhang, Yu,Lin, Zhixin,Sun, Zongxiu,Qian, Qian,Lu, Yuping,Han, Bin.

[9]Comparison of transcriptomes undergoing waterlogging at the seedling stage between tolerant and sensitive varieties of Brassica napus L.. Zou Xi-ling,Zeng Liu,Lu Guang-yuan,Cheng yong,Xu Jin-song,Zhang Xue-kun. 2015

[10]Comprehensive analysis of expressed sequence tags from cultivated and wild radish (Raphanus spp.). Shen, Di,Qiu, Yang,Li, Xixiang,Shen, Di,Sun, Honghe,Huang, Mingyun,Zheng, Yi,Fei, Zhangjun,Sun, Honghe,Fei, Zhangjun. 2013

[11]Comparative genome analysis of the oleaginous yeast Trichosporon fermentans reveals its potential applications in lipid accumulation. Shen, Qi,Wang, Qun,Zhao, Yu-Hua,Chen, Yue,Jin, Danfeng,Lin, Hui.

[12]Gas Chromatography-Mass Spectrometric Analysis of Nematicidal Essential Oil of Valeriana amurensis P Smirn ex Kom (Valerianaceae) Roots and its Activity against Heterodera avenae. Li, Yun Chao,Ji, Hua,Li, Hong Tao. 2015

[13]Large-Scale Identification and Characterization of Heterodera avenae Putative Effectors Suppressing or Inducing Cell Death in Nicotiana benthamiana. Chen, Changlong,Chen, Yongpan,Jian, Heng,Yang, Dan,Dai, Yiran,Pan, Lingling,Shi, Fengwei,Yang, Shanshan,Liu, Qian,Chen, Changlong,Pan, Lingling,Shi, Fengwei. 2018

[14]Isolation of nematicidal constituents from essential oil of Kaempferia galanga L rhizome and their activity against Heterodera avenae Wollenweber. Li, Yun Chao,Ji, Hua,Li, Hong Tao,Li, Yun Chao,Ji, Hua,Li, Hong Tao,Li, Xiu Hua,Zhang, Hai Xin. 2017

[15]Testing and modelling the potential of three diploid plants in Poaceae as a new pathosystem to investigate the interactions between cereal hosts and cereal cyst nematode (Heterodera avenae). Wu, D. Q.,Cui, J. K.,Huang, W. K.,Peng, H.,Peng, D. L..

[16]Golden Promise barley (Hordeum vulgare) is a suitable candidate model host for investigation interaction with Heterodera avenae. Kong Ling-an,Peng Huan,Huang Wen-kun,Cui Jiang-kuan,Liu Jing,Qiao Fen,Peng De-liang,Luo Shu-jie,Jian Heng. 2017

[17]GC-MS Analysis of Nematicidal Essential Oil of Mentha canadensis Aerial Parts against Heterodera avenae and Meloidogyne incognita. Ji, Hua,Li, Yun Chao,Wen, Zhi Yu,Li, Hong Tao,Ji, Hua,Li, Yun Chao,Wen, Zhi Yu,Li, Hong Tao,Li, Xiu Hua,Zhang, Hai Xin.

[18]Host status of Brachypodium distachyon to the cereal cyst nematode. Chen Chang-long,Liu Shu-sen,Liu Qian,Liu Pei,Zhao Jian-long,Jian Heng,Chen Chang-long,Li Hong-jie,Liu Shu-sen,Niu Jun-hai,Liu Zhi-yong,Liu Zhi-yong. 2018

[19]A new pathotype characterization of Daxing and Huangyuan populations of cereal cyst nematode (Heterodera avenae) in China. Huang Wen-kun,Peng Huan,Liu Shi-ming,Wang Gao-feng,Kong Lin-an,Peng De-liang. 2015

[20]Large-scale identification of wheat genes resistant to cereal cyst nematode Heterodera avenae using comparative transcriptomic analysis. Wu, Du-Qing,Huang, Wen-Kun,Peng, Huan,Wang, Gao-Feng,Cui, Jiang-Kuan,Liu, Shi-Ming,Peng, De-Liang,Li, Zhi-Gang,Yang, Jun,Li, Zhi-Gang,Yang, Jun. 2015

作者其他论文 更多>>

微信小程序