Jasmonate-sensitivity-assisted screening and characterization of nicotine synthetic mutants from activation-tagged population of tobacco (Nicotiana tabacum L.)

文献类型： 外文期刊

第一作者： Yin, Guoying

作者： Yin, Guoying;Niu, Haixia;Ding, Yongqiang;Zhang, Dingyu;Zhang, Jie;Wang, Sangen;Wang, Wenjing;Liu, Guanshan;Zhang, Hongbo

作者机构：

关键词： tobacco;activation-tagged population;nictine;mutant;jasmonate-sensitivity

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.753； 五年影响因子：6.612 ）

ISSN： 1664-462X

年卷期： 2017 年 8 卷

页码：

收录情况： SCI

摘要： Nicotine is a secondary metabolite that is important to the defense system and commercial quality of tobacco (Nicotiana tabacum L.). Jasmonate and its derivatives (JAs) are phytohormone regulators of nicotine formation; however, the underlying molecular mechanism of this process remains largely unclear. Owing to the amphitetraploid origin of N. tabacum, research on screening and identification of nicotine-synthetic mutants is relatively scarce. Here, we describe a method based on JA-sensitivity for screening nicotine mutants from an activation-tagged population of tobacco. In this approach, the mutants were first screened for abnormal JA responses in seed germination and root elongation, and then the levels of nicotine synthesis and expression of nicotine synthetic genes in the mutants with altered JA-response were measured to determine the nicotine-synthetic mutants. We successfully obtained five mutants thatmaintained stable nicotine contents and JA responses for three generations. This method is simple, effective and low-cost, and the finding of transcriptional changes of nicotine synthetic genes in themutants shows potentials for identifying novel regulators involved in JA-regulated nicotine biosynthesis.

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