Adaptation response of Pseudomonas fragi on refrigerated solid matrix to a moderate electric field

文献类型： 外文期刊

第一作者： Chen, Wenbo

作者： Chen, Wenbo;Hu, Honghai;Zhang, Chunjiang;Huang, Feng;Zhang, Dequan;Zhang, Hong;Chen, Wenbo;Hu, Honghai;Zhang, Chunjiang;Huang, Feng;Zhang, Hong

作者机构：

关键词： Adaptation response;Moderate electric field;Nutrient agar plates;Psychrotrophic microorganisms;Pseudomonas fragi;Solid cellular foodstuffs

期刊名称：BMC MICROBIOLOGY （ 影响因子：3.605； 五年影响因子：4.283 ）

ISSN： 1471-2180

年卷期： 2017 年 17 卷

页码：

收录情况： SCI

摘要： Background: Moderate electric field (MEF) technology is a promising food preservation strategy since it relies on physical properties-rather than chemical additives-to preserve solid cellular foods during storage. However, the effectiveness of long-term MEF exposure on the psychrotrophic microorganisms responsible for the food spoilage at cool temperatures remains unclear. Results: The spoilage-associated psychrotroph Pseudomonas fragi MC16 was obtained from pork samples stored at 7 degrees C. Continuous MEF treatment attenuated growth and resulted in subsequent adaptation of M16 cultured on nutrient agar plates at 7 degrees C, compared to the control cultures, as determined by biomass analysis and plating procedures. Moreover, intracellular dehydrogenase activity and ATP levels also indicated an initial effect of MEF treatment followed by cellular recovery, and extracellular beta-galactosidase activity assays indicated no obvious changes in cell membrane permeability. Furthermore, microscopic observations using scanning and transmission electron microscopy revealed that MEF induced sublethal cellular injury during early treatment stages, but no notable changes in morphology or cytology on subsequent days. Conclusion: Our study provides direct evidence that psychrotrophic P. fragi MC16 cultured on nutrient agar plates at 7 degrees C are capable of adapting to MEF treatment.

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