LCN2 regulates the gut microbiota and metabolic profile in mice infected with Mycobacterium bovis

文献类型： 外文期刊

第一作者： Huang, Quntao

作者： Huang, Quntao;Liu, Mengting;Gao, Mengtian;Wang, Jingwen;Tang, Fang;Ren, Jianluan;Zhao, Chengzhu;Wang, Xinru;Zhou, Xinyu;Luo, Haodong;Dai, Jianjun;Xue, Feng;Xing, Junhong;Li, Guoli;Yu, Youli;Zeng, Dexin;Zeng, Dexin;Dai, Jianjun

作者机构：

关键词： Lcn2; M. bovis; gut microbiota; non-targeted metabolome

期刊名称：MSYSTEMS （ 影响因子：5.0； 五年影响因子：6.1 ）

ISSN： 2379-5077

年卷期： 2024 年 9 卷 8 期

页码：

收录情况： SCI

摘要： Infection with Mycobacterium bovis precipitates a spectrum of pathologies in bovines, notably necrotic pneumonia, mastitis, and arthritis, impinging upon the health and nutritional assimilation of these animals. A pivotal factor, lipocalin 2 (Lcn2), is responsive to microbial invasion, inflammatory processes, and tissue damage, the extent of which Lcn2 modulates the gut environment, however, remains unclear in response to M. bovis-induced alterations. To explore the role of Lcn2 in shaping the gut milieu of mice during a 5-week period post-M. bovis infection, Lcn2 knockout Lcn2(-/-) mice were scrutinized for changes in the gut microbiota and metabolomic profiles. Results showed that Lcn2(-/-) mice infected with M. bovis exhibited notable shifts in the operational taxonomic units (OTUs) of gut microbiota, alongside significant disparities in alpha and beta diversity. Concomitantly, a marked increase was observed during the 5-week period in the abundance of Akkermansia, Oscillospira, and Bacteroides, coupled with a substantial decrease in Ruminococcus within the microbiome of Lcn2 knockout mice. Notably, Akkermansia muciniphila was significantly enriched in the gut flora of Lcn2(-/-) mice. Furthermore, the absence of Lcn2 significantly altered the gut metabolomic landscape, evidenced by elevated levels of metabolites such as taurodeoxycholic acid, 10-undecenoic acid, azelaic acid, and dodecanedioic acid in Lcn2(-/-) mice. Our findings demonstrated that the lack of Lcn2 in the context of M. bovis infection profoundly affected the regulation of gut microbiota and metabolomic components, culminating in a transformed gut environment. Our results revealed that Lcn2 may regulate gut microbiota and metabolome components, changing the intestinal environment, thereby affecting the infection status of M. bovis.

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